Chicken Ubiquitin Polyclonal Antibody | anti-UBC antibody

Ubiquitin Chicken Polyclonal Antibody

Cat. Num. AAA588285

• Gene Names: UBC; HMG20
• Synonyms: Ubiquitin; Polyclonal Antibody; Ubiquitin Chicken Polyclonal Antibody; anti-UBC antibody

• Host: Chicken
• Clonality: Polyclonal
• Form/Format: Lyophilized purified total IgY in PBS pH7.4
• Sequence Length: 685

• Preservative: Ampicillin and streptomycin, 0.05% sodium aside.
• Preparation and Storage: Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.; To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).; For maximum recovery of product centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Paraffin embedded pig artery tissue section was stained for ubiquitin presence with the anti-chicken IgY. The reaction was visualized with anti-chicken antibody (red), DNA was stained with DAPI (blue) and elastin layer is stained in green. Ubiquitin is Shown in significant nuclear staining in the upper smooth muscle cell layer as well as in the endothelial cells.)

NCBI and Uniprot Product Information

• NCBI GI #: 340068
• NCBI GeneID: 7316
• Molecular Weight: 77,039 Da
• NCBI Official Full Name: ubiquitin
• NCBI Official Synonym Full Names: ubiquitin C
• NCBI Official Symbol: UBC
• NCBI Official Synonym Symbols: HMG20
• NCBI Protein Information: polyubiquitin-C
• UniProt Protein Name: Polyubiquitin-C
• Protein Family: Ubiquitin
• UniProt Gene Name: UBC

NCBI Description

This gene represents a ubiquitin gene, ubiquitin C. The encoded protein is a polyubiquitin precursor. Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell, depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, endocytosis, and regulation of other cell signaling pathways. [provided by RefSeq, Aug 2010]

Uniprot Description

Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.