PIGM sirna

PIGM siRNA (Mouse)

Cat. Num. AAA8204332

• Gene Names: Pigm; AI644422; AV302080; 4933437L05Rik; C920011G20Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PIGM; PIGM siRNA (Mouse); GPI mannosyltransferase 1; GPI mannosyltransferase I; GPI-MT-I; Phosphatidylinositol-glycan biosynthesis class M protein; PIG-M; PIGM sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PIGM siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 423

• Applicable Applications for PIGM sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PIGM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PIGM sirna

siRNA to inhibit PIGM expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19920329
• NCBI GeneID: 67556
• NCBI Accession #: NP_080510.1
• NCBI GenBank Nucleotide #: NM_026234.4
• UniProt Accession #: Q8C2R7; Q8C917; Q99J22; Q9D315
• Molecular Weight: 49,789 Da
• NCBI Official Full Name: GPI mannosyltransferase 1
• NCBI Official Synonym Full Names: phosphatidylinositol glycan anchor biosynthesis, class M
• NCBI Official Symbol: Pigm
• NCBI Official Synonym Symbols: AI644422; AV302080; 4933437L05Rik; C920011G20Rik
• NCBI Protein Information: GPI mannosyltransferase 1
• UniProt Protein Name: GPI mannosyltransferase 1
• Protein Family: Pigment-dispersing hormone
• UniProt Gene Name: Pigm
• UniProt Synonym Gene Names: GPI-MT-I; PIG-M
• UniProt Entry Name: PIGM_MOUSE

Uniprot Description

PIGM: Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Defects in PIGM are the cause of glycosylphosphatidylinositol deficiency (GPID). GPID is an autosomal recessive trait that results in a propensity to venous thrombosis and seizures. Deficiency is due to a point mutation in the regulatory sequences of PIGM that disrupts binding of the transcription factor SP1 to its cognate promoter motif, leading to a strong reduction of expression. Belongs to the PIGM family.

Protein type: Transferase; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; EC 2.4.1.-; Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: acetylgalactosaminyltransferase activity; oligosaccharyl transferase activity; alpha(1,6)-fucosyltransferase activity; alpha(1,3)-fucosyltransferase activity; glucosyltransferase activity; O antigen polymerase activity; alpha-1,2-mannosyltransferase activity; acetylglucosaminyltransferase activity; transferase activity, transferring glycosyl groups; 1-phenanthrol glycosyltransferase activity; phenanthrol glycosyltransferase activity; glycolipid mannosyltransferase activity; galactosyltransferase activity; dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity; fucosyltransferase activity; dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity; lipopolysaccharide-1,6-galactosyltransferase activity; alpha-1,2-galactosyltransferase activity; alpha-1,3-galactosyltransferase activity; 9-phenanthrol UDP-glucuronosyltransferase activity; dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; transferase activity, transferring hexosyl groups; UDP-glucose:glycoprotein glucosyltransferase activity; 1,2-dihydroxy-phenanthrene glycosyltransferase activity; 9-phenanthrol glycosyltransferase activity; dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; transferase activity; beta-1,4-mannosyltransferase activity; cellulose synthase activity; mannosyltransferase activity; alpha-1,3-mannosyltransferase activity; alpha-1,6-mannosyltransferase activity; inositol phosphoceramide synthase activity

Biological Process: GPI anchor biosynthetic process