TBX1 sirna

TBX1 siRNA (Mouse)

Cat. Num. AAA8204330

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TBX1; TBX1 siRNA (Mouse); T-box transcription factor TBX1; T-box protein 1; Testis-specific T-box protein; TBX1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TBX1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 488

• Applicable Applications for TBX1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TBX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TBX1 sirna

siRNA to inhibit TBX1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22094109
• NCBI GeneID: 21380
• NCBI Accession #: NP_035662.1
• NCBI GenBank Nucleotide #: NM_011532.2
• UniProt Accession #: P70323; Q60706; Q99MP0; Q99P22
• Molecular Weight: 52,507 Da
• NCBI Official Full Name: T-box transcription factor TBX1 isoform 1
• NCBI Official Synonym Full Names: T-box 1
• NCBI Official Symbol: Tbx1
• NCBI Protein Information: T-box transcription factor TBX1
• UniProt Protein Name: T-box transcription factor TBX1
• Protein Family: T-box transcription factor
• UniProt Gene Name: Tbx1
• UniProt Synonym Gene Names: T-box protein 1
• UniProt Entry Name: TBX1_MOUSE

Uniprot Description

TBX1: Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries. Haploinsufficiency of the TBX1 gene is responsible for most of the physical malformations present in DiGeorge syndrome (DGS) and velocardiofacial syndrome (VCFS). DGS is characterized by the association of several malformations: hypoplastic thymus and parathyroid glands, congenital conotruncal cardiopathy, and a subtle but characteristic facial dysmorphology. VCFS is marked by the association of congenital conotruncal heart defects, cleft palate or velar insufficiency, facial dysmorpholgy and learning difficulties. It is now accepted that these two syndromes represent two forms of clinical expression of the same entity manifesting at different stages of life. Defects in TBX1 are a cause of DiGeorge syndrome (DGS). Defects in TBX1 are a cause of velocardiofacial syndrome (VCFS). Defects in TBX1 are a cause of conotruncal heart malformations (CTHM). CTHM consist of cardiac outflow tract defects, such as tetralogy of Fallot, pulmonary atresia, double-outlet right ventricle, truncus arteriosus communis, and aortic arch anomalies. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Cellular Component: nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: heart morphogenesis; muscle development; positive regulation of transcription, DNA-dependent; heart development; multicellular organismal development; muscle cell fate commitment; middle ear morphogenesis; anterior/posterior pattern formation; positive regulation of mesenchymal cell proliferation; mesoderm development; muscle morphogenesis; neural crest cell migration; inner ear morphogenesis; tongue morphogenesis; transcription, DNA-dependent; outer ear morphogenesis; semicircular canal morphogenesis; embryonic cranial skeleton morphogenesis; social behavior; parathyroid gland development; patterning of blood vessels; regulation of transcription from RNA polymerase II promoter; blood vessel morphogenesis; positive regulation of transcription from RNA polymerase II promoter; lymph vessel development; determination of left/right symmetry; positive regulation of epithelial cell proliferation; transcription from RNA polymerase II promoter; retinoic acid receptor signaling pathway; cell fate specification; ear morphogenesis; negative regulation of transcription from RNA polymerase II promoter; sensory perception of sound; regulation of transcription, DNA-dependent; positive regulation of MAPKKK cascade; epithelial cell differentiation; positive regulation of cell proliferation; thyroid gland development; angiogenesis; blood vessel development; pharyngeal system development; thymus development; embryonic viscerocranium morphogenesis; pattern specification process; odontogenesis of dentine-containing teeth; cell proliferation; negative regulation of cell differentiation; artery morphogenesis; blood vessel remodeling; positive regulation of protein amino acid phosphorylation; soft palate development; vagus nerve morphogenesis