Western Blot (WB)
(Western blot analysis of Adenylate Cyclase 5/6 expression in HeLa (A), Raw264.7 (B), PC12 (C), rat brain (D) whole cell lysates.)
Rabbit Adenylate Cyclase 5/6 Polyclonal Antibody | anti-ADCY5 antibody
Anti-Adenylate Cyclase 5/6 Antibody
Western Blot (WB)
(Western blot analysis of Adenylate Cyclase 5/6 expression in HeLa (A), Raw264.7 (B), PC12 (C), rat brain (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Adenylate Cyclase 5/6 expression in HeLa (A), Raw264.7 (B), PC12 (C), rat brain (D) whole cell lysates.)
Immunohistochemistry (IHC)
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Immunohistochemistry (IHC)
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Immunofluorescence (IF)
(Immunofluorescent analysis of Adenylate Cyclase 5/6 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of Adenylate Cyclase 5/6 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the membrane-bound adenylyl cyclase enzymes. Adenylyl cyclases mediate G protein-coupled receptor signaling through the synthesis of the second messenger cAMP. Activity of the encoded protein is stimulated by the Gs alpha subunit of G protein-coupled receptors and is inhibited by protein kinase A, calcium and Gi alpha subunits. Single nucleotide polymorphisms in this gene may be associated with low birth weight and type 2 diabetes. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
Uniprot Description
ADCY5: This is a membrane-bound, calcium-inhibitable adenylyl cyclase. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Lyase; EC 4.6.1.1; Nucleotide Metabolism - purine; Transporter; Membrane protein, integral; Adenylyl cyclase; Transporter, aquaporin family
Chromosomal Location of Human Ortholog: 3q21.1
Cellular Component: plasma membrane; integral to membrane
Molecular Function: protein heterodimerization activity; metal ion binding; ATP binding; adenylate cyclase activity; adenylate cyclase binding
Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; locomotory behavior; signal transduction; G-protein signaling, adenylate cyclase activating pathway; dopamine receptor, adenylate cyclase inhibiting pathway; synaptic transmission; phospholipase C activation; energy reserve metabolic process; innate immune response; renal water homeostasis; cAMP biosynthetic process; G-protein signaling, adenylate cyclase inhibiting pathway; neuromuscular process controlling balance; transmembrane transport; adenosine receptor signaling pathway; dopamine receptor, adenylate cyclase activating pathway
Disease: Dyskinesia, Familial, With Facial Myokymia
Research Articles on ADCY5
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Product Notes
The ADCY5 adcy5 (Catalog #AAA820420) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Adenylate Cyclase 5/6 Antibody reacts with Human, Mouse, Rat, Dog, Monkey, Rabbit, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's Adenylate Cyclase 5/6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC). WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500). Researchers should empirically determine the suitability of the ADCY5 adcy5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adenylate Cyclase 5/6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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