Typical Testing Data/Standard Curve (for reference only)
Rat Adenylate cyclase type 5 (ADCY5) ELISA Kit | ADCY5 elisa kit
Rat Adenylate cyclase type 5 (ADCY5) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ADCY2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADCY2 antibody and an ADCY2-HRP conjugate. The assay sample and buffer are incubated together with ADCY2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADCY2 concentration since ADCY2 from samples and ADCY2-HRP conjugate compete for the anti-ADCY2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADCY2 from the sample, fewer sites are left to bind ADCY2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADCY2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the membrane-bound adenylyl cyclase enzymes. Adenylyl cyclases mediate G protein-coupled receptor signaling through the synthesis of the second messenger cAMP. Activity of the encoded protein is stimulated by the Gs alpha subunit of G protein-coupled receptors and is inhibited by protein kinase A, calcium and Gi alpha subunits. Single nucleotide polymorphisms in this gene may be associated with low birth weight and type 2 diabetes. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
Uniprot Description
ADCY5: This is a membrane-bound, calcium-inhibitable adenylyl cyclase. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 4.6.1.1; Adenylyl cyclase; Membrane protein, multi-pass; Nucleotide Metabolism - purine; Transporter; Membrane protein, integral; Transporter, aquaporin family; Lyase
Chromosomal Location of Human Ortholog: 3q21.1
Cellular Component: plasma membrane; integral to membrane
Molecular Function: protein heterodimerization activity; metal ion binding; ATP binding; adenylate cyclase activity; adenylate cyclase binding
Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; locomotory behavior; signal transduction; G-protein signaling, adenylate cyclase activating pathway; dopamine receptor, adenylate cyclase inhibiting pathway; synaptic transmission; phospholipase C activation; energy reserve metabolic process; innate immune response; renal water homeostasis; cAMP biosynthetic process; G-protein signaling, adenylate cyclase inhibiting pathway; neuromuscular process controlling balance; adenosine receptor signaling pathway; transmembrane transport; dopamine receptor, adenylate cyclase activating pathway
Disease: Dyskinesia, Familial, With Facial Myokymia
Research Articles on ADCY5
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Product Notes
The Rat ADCY5 adcy5 (Catalog #AAA7206841) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7206841 ELISA Kit recognizes Rat ADCY5. It is sometimes possible for the material contained within the vial of "Adenylate cyclase type 5 (ADCY5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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