Western Blot (WB)
(Western blot analysis of ARF4 expression in HeLa (A), HepG2 (B), mouse liver (C), mouse kidney (D), rat kidney (E) whole cell lysates.)
Rabbit ARF4 Polyclonal Antibody | anti-ARF4 antibody
Anti-ARF4 Antibody
Western Blot (WB)
(Western blot analysis of ARF4 expression in HeLa (A), HepG2 (B), mouse liver (C), mouse kidney (D), rat kidney (E) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of ARF4 expression in HeLa (A), HepG2 (B), mouse liver (C), mouse kidney (D), rat kidney (E) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of ARF4 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of ARF4 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ARF4: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family.
Protein type: Motility/polarity/chemotaxis; G protein; G protein, monomeric; G protein, monomeric, ARF
Chromosomal Location of Human Ortholog: 3p14.3
Cellular Component: Golgi apparatus; membrane; cytosol
Molecular Function: GTPase activity; protein binding; GTP binding; epidermal growth factor receptor binding
Biological Process: vesicle-mediated transport; epidermal growth factor receptor signaling pathway; phospholipase D activation; cell migration; protein transport; protein amino acid ADP-ribosylation; small GTPase mediated signal transduction; organelle organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; brain development; response to axon injury; negative regulation of apoptosis
Research Articles on ARF4
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Product Notes
The ARF4 arf4 (Catalog #AAA8204121) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ARF4 Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's ARF4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB (1/500 - 1/1000), IHC (1/100 - 1/200), IP (1/10 - 1/100). Researchers should empirically determine the suitability of the ARF4 arf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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