ZMYND8 sirna

ZMYND8 siRNA (Human)

Cat. Num. AAA8203566

• Gene Names: ZMYND8; RACK7; PRKCBP1; PRO2893
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZMYND8; ZMYND8 siRNA (Human); KIAA1125; PRKCBP1; RACK7; Protein kinase C-binding protein 1; Cutaneous T-cell lymphoma-associated antigen se14-3; CTCL-associated antigen se14-3; Rack7; Zinc finger MYND domain-containing protein 8; ZMYND8 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZMYND8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1054

• Applicable Applications for ZMYND8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZMYND8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZMYND8 sirna

siRNA to inhibit ZMYND8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 528881103
• NCBI GeneID: 23613
• NCBI Accession #: NP_001268700.1
• NCBI GenBank Nucleotide #: NM_001281771.2
• UniProt Accession #: Q9ULU4; Q13517; Q2HXV1; B3KVL2; B7Z2A8; B7Z3E0; B7Z680; B7ZM62; E1P5U5; F5H0X3; H7C0U2; J3KPU3
• Molecular Weight: 122,116 Da
• NCBI Official Full Name: protein kinase C-binding protein 1 isoform e
• NCBI Official Synonym Full Names: zinc finger, MYND-type containing 8
• NCBI Official Symbol: ZMYND8
• NCBI Official Synonym Symbols: RACK7; PRKCBP1; PRO2893
• NCBI Protein Information: protein kinase C-binding protein 1
• UniProt Protein Name: Protein kinase C-binding protein 1
• Protein Family: Protein kinase C-binding protein
• UniProt Gene Name: ZMYND8
• UniProt Synonym Gene Names: KIAA1125; PRKCBP1; RACK7; CTCL-associated antigen se14-3
• UniProt Entry Name: PKCB1_HUMAN

NCBI Description

The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RACK7: a DNA damage response (DDR) protein, receptor for activated C-kinase (RACK) protein, and a transcriptional regulator. A bromodomain (BRD)-containing protein that recruits the nucleosome remodeling and histone deacetylation (NuRD) complex to damaged chromatin. Targets DNA damage within actively transcribed regions, repressing transcription, and promoting repair by homologous recombination. A cutaneous T-cell lymphoma-associated antigen that contains a bromodomain and two zinc fingers. Twenty-two alternatively spliced isoforms of the human protein hve been reported. Note: This description may include information from UniProtKB

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: nucleus

Molecular Function: protein binding; zinc ion binding

Biological Process: negative regulation of transcription from RNA polymerase II promoter