HACE1 sirna

HACE1 siRNA (Human)

Cat. Num. AAA8203447

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HACE1; HACE1 siRNA (Human); KIAA1320; E3 ubiquitin-protein ligase HACE1; HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; HACE1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HACE1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 909

• Applicable Applications for HACE1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HACE1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HACE1 sirna

siRNA to inhibit HACE1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 116875852
• NCBI GeneID: 57531
• NCBI Accession #: NP_065822.2
• NCBI GenBank Nucleotide #: NM_020771.3
• UniProt Accession #: Q8IYU2; Q5VU99; Q5VUA0; Q8ND12; Q9P2M6; A8K6U5; B3KY89; B4DFM6; B4DTQ4; B7Z9X6; E9PGP0
• Molecular Weight: 77,963 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase HACE1
• NCBI Official Synonym Full Names: HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1
• NCBI Official Symbol: HACE1
• NCBI Protein Information: E3 ubiquitin-protein ligase HACE1
• UniProt Protein Name: E3 ubiquitin-protein ligase HACE1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: HACE1
• UniProt Synonym Gene Names: KIAA1320
• UniProt Entry Name: HACE1_HUMAN

Uniprot Description

HACE1: E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB. Defects in HACE1 are a cause of Wilms tumor (WT). WT is a pediatric malignancy of kidney and one of the most common solid cancers in childhood. HACE1 is epigenetically down-regulated in sporadic Wilms tumor. Moreover, a t(5;6)(q21;q21) translocation that truncates HACE1 has been found in a child with bilateral, young-onset Wilms tumor. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 6q16.3

Cellular Component: Golgi membrane; endoplasmic reticulum; nucleus

Molecular Function: protein binding; Rac GTPase binding; ubiquitin-protein ligase activity; Rab GTPase binding; ligase activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; protein ubiquitination during ubiquitin-dependent protein catabolic process; Rac protein signal transduction; protein ubiquitination; cell cycle; regulation of cell migration; Golgi organization and biogenesis