SLC4A1 sirna

SLC4A1 siRNA (Rat)

Cat. Num. AAA8203377

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC4A1; SLC4A1 siRNA (Rat); AE1; Band 3 anion transport protein; Anion exchange protein 1; AE 1; Anion exchanger 1; Solute carrier family 4 member 1; CD antigen CD233; SLC4A1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SLC4A1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 928

• Applicable Applications for SLC4A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SLC4A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC4A1 sirna

siRNA to inhibit SLC4A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 76443687
• NCBI GeneID: 24779
• NCBI Accession #: NP_036783.2
• NCBI GenBank Nucleotide #: NM_012651.2
• UniProt Accession #: P23562
• Molecular Weight: 94,153 Da
• NCBI Official Full Name: band 3 anion transport protein
• NCBI Official Synonym Full Names: solute carrier family 4 (anion exchanger), member 1
• NCBI Official Symbol: Slc4a1
• NCBI Protein Information: band 3 anion transport protein
• UniProt Protein Name: Band 3 anion transport protein
• Protein Family: Band 3 anion exchange protein
• UniProt Gene Name: Slc4a1
• UniProt Synonym Gene Names: Ae1; AE 1; Anion exchanger 1
• UniProt Entry Name: B3AT_RAT

NCBI Description

a transporter; functions as a chloride/carbonate exchanger and binds to components of the cytoskeleton [RGD, Feb 2006]

Uniprot Description

SLC4A1: Band 3 is the major integral glycoprotein of the erythrocyte membrane. Band 3 has two functional domains. Its integral domain mediates a 1:1 exchange of inorganic anions across the membrane, whereas its cytoplasmic domain provides binding sites for cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Defects in SLC4A1 are the cause of elliptocytosis type 4 (EL4). EL4 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape. Defects in SLC4A1 are the cause of spherocytosis type 4 (SPH4); also known as hereditary spherocytosis type 4 (HS4). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Defects in SLC4A1 are the cause of renal tubular acidosis, distal, autosomal dominant (AD-dRTA). A disease characterized by reduced ability to acidify urine, variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Defects in SLC4A1 are the cause of renal tubular acidosis, distal, with hemolytic anemia (dRTA-HA). A disease characterized by the association of hemolytic anemia with distal renal tubular acidosis, the reduced ability to acidify urine resulting in variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Defects in SLC4A1 are the cause of renal tubular acidosis, distal, with normal red cell morphology (dRTA-NRC). A disease characterized by reduced ability to acidify urine, variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Belongs to the anion exchanger (TC 2.A.31) family.

Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, SLC family

Cellular Component: cortical cytoskeleton; membrane; basolateral plasma membrane; integral to plasma membrane; Z disc

Molecular Function: protein C-terminus binding; bicarbonate transmembrane transporter activity; protein homodimerization activity; inorganic anion exchanger activity; chloride transmembrane transporter activity; anion:anion antiporter activity; anion transmembrane transporter activity; ankyrin binding; actin binding

Biological Process: bicarbonate transport; regulation of intracellular pH; chloride transport; anion transport