ATRX sirna

ATRX siRNA (Mouse)

Cat. Num. AAA8203025

• Gene Names: Atrx; XH2; Xnp; ATR2; MRXS3; Rad54; Hp1bp2; RAD54L; ZNF-HX; Hp1bp38; AI447451; HP1-BP38; DXHXS6677E; 4833408C14Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATRX; ATRX siRNA (Mouse); HP1BP2; XNP; Transcriptional regulator ATRX; ATP-dependent helicase ATRX; HP1 alpha-interacting protein; HP1-BP38 protein; Heterochromatin protein 2; X-linked nuclear protein; ATRX sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ATRX siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2476

• Applicable Applications for ATRX sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ATRX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATRX sirna

siRNA to inhibit ATRX expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 154091016
• NCBI GeneID: 22589
• NCBI Accession #: NP_033556.2
• NCBI GenBank Nucleotide #: NM_009530.2
• UniProt Accession #: Q61687; A2ADH4
• Molecular Weight: 278,587 Da
• NCBI Official Full Name: transcriptional regulator ATRX
• NCBI Official Synonym Full Names: alpha thalassemia/mental retardation syndrome X-linked homolog (human)
• NCBI Official Symbol: Atrx
• NCBI Official Synonym Symbols: XH2; Xnp; ATR2; MRXS3; Rad54; Hp1bp2; RAD54L; ZNF-HX; Hp1bp38; AI447451; HP1-BP38; DXHXS6677E; 4833408C14Rik
• NCBI Protein Information: transcriptional regulator ATRX
• UniProt Protein Name: Transcriptional regulator ATRX
• Protein Family: Transcriptional regulator
• UniProt Gene Name: Atrx
• UniProt Synonym Gene Names: Hp1bp2; Xnp
• UniProt Entry Name: ATRX_MOUSE

Uniprot Description

ATRX: a global transcriptional regulator. Belongs to the SNF2 family of proteins, many of which modify gene expression via chromatin remodeling activity. Involved in the developmental silencing of imprinted genes in the brain. Contains one PxVxL motif, which is required for interaction with chromoshadow domains. This motif requires additional residues at -7, -6, +4 and +5 relative to the central V which contact the chromoshadow domain. Constitutive mutations in ATRX are associated with brain, facial, and genital abnormalities, and alpha thalassemia. Acquired mutations in ATRX have been observed in preleukemic conditions. Six alternatively spliced human isoforms have been described.

Protein type: EC 3.6.4.12; Helicase; DNA repair, damage; Ubiquitin conjugating system

Cellular Component: nucleoplasm; heterochromatin; nuclear chromosome; chromosome, telomeric region; cytoplasm; chromosome; intracellular; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; hydrolase activity; histone binding; metal ion binding; nucleotide binding; chromatin binding; helicase activity; DNA translocase activity; methylated histone residue binding; ATP binding

Biological Process: transcription, DNA-dependent; DNA damage response, signal transduction by p53 class mediator; multicellular organism growth; positive regulation of telomere maintenance; chromatin modification; DNA repair; Sertoli cell development; replication fork processing; chromatin remodeling; nucleosome assembly; regulation of transcription, DNA-dependent; DNA replication-independent nucleosome assembly; post-embryonic forelimb morphogenesis; forebrain development; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus