NCF1 sirna

NCF1 siRNA (Human)

Cat. Num. AAA8202067

• Gene Names: NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NCF1; NCF1 siRNA (Human); NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox; NCF1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NCF1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 390

• Applicable Applications for NCF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NCF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NCF1 sirna

siRNA to inhibit NCF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 528078313
• NCBI GeneID: 653361
• NCBI Accession #: NP_000256.4
• NCBI GenBank Nucleotide #: NM_000265.5
• UniProt Accession #: P14598; O43842; Q2PP07; Q53FR5; Q9BU90; Q9BXI7; Q9BXI8; Q9UDV9; Q9UMU2; A6NEH2; A8K7S9
• Molecular Weight: 30,781 Da
• NCBI Official Full Name: neutrophil cytosol factor 1
• NCBI Official Synonym Full Names: neutrophil cytosolic factor 1
• NCBI Official Symbol: NCF1
• NCBI Official Synonym Symbols: NCF1A; NOXO2; p47phox; SH3PXD1A
• NCBI Protein Information: neutrophil cytosol factor 1
• UniProt Protein Name: Neutrophil cytosol factor 1
• Protein Family: Neutrophil cytosol factor
• UniProt Gene Name: NCF1
• UniProt Synonym Gene Names: NOXO2; SH3PXD1A; NCF-1
• UniProt Entry Name: NCF1_HUMAN

NCBI Description

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]

Uniprot Description

p47phox: the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.

Protein type: EC 1.-.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: Golgi apparatus; extrinsic to membrane; rough endoplasmic reticulum; cell soma; dendrite; cytosol; NADPH oxidase complex

Molecular Function: protein binding; electron carrier activity; superoxide-generating NADPH oxidase activity; phosphatidylinositol-3,4-bisphosphate binding; SH3 domain binding; phosphoinositide binding

Biological Process: respiratory burst during defense response; respiratory burst; interaction with host; apoptosis; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; leukotriene metabolic process; cell proliferation; antigen processing and presentation of peptide antigen via MHC class I; negative regulation of smooth muscle contraction; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; cellular defense response; response to yeast; protein targeting to membrane; vascular endothelial growth factor receptor signaling pathway; inflammatory response; superoxide release; hydrogen peroxide biosynthetic process

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type I