SREBF1 sirna

SREBF1 siRNA (Mouse)

Cat. Num. AAA8202014

• Gene Names: Srebf1; ADD1; ADD-1; SREBP1; bHLHd1; SREBP-1; SREBP1c; SREBP-1a; SREBP-1c; D630008H06
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SREBF1; SREBF1 siRNA (Mouse); SREBP1; Sterol regulatory element-binding protein 1; SREBP-1; Sterol regulatory element-binding transcription factor 1; SREBF1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SREBF1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1134

• Applicable Applications for SREBF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SREBF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SREBF1 sirna

siRNA to inhibit SREBF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 27753981
• NCBI GeneID: 20787
• NCBI Accession #: NP_035610.1
• NCBI GenBank Nucleotide #: NM_011480.3
• UniProt Accession #: Q9WTN3; Q3U458; Q3UDJ3; Q5SRX5; Q8C733; Q99JK7
• Molecular Weight: 113,734 Da
• NCBI Official Full Name: sterol regulatory element-binding protein 1
• NCBI Official Synonym Full Names: sterol regulatory element binding transcription factor 1
• NCBI Official Symbol: Srebf1
• NCBI Official Synonym Symbols: ADD1; ADD-1; SREBP1; bHLHd1; SREBP-1; SREBP1c; SREBP-1a; SREBP-1c; D630008H06
• NCBI Protein Information: sterol regulatory element-binding protein 1
• UniProt Protein Name: Sterol regulatory element-binding protein 1
• UniProt Gene Name: Srebf1
• UniProt Synonym Gene Names: Srebp1; SREBP-1
• UniProt Entry Name: SRBP1_MOUSE

NCBI Description

This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

SREBP-1: Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle. Belongs to the SREBP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Membrane protein, multi-pass; Membrane protein, integral; DNA-binding

Cellular Component: Golgi apparatus; protein complex; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; cytoplasm; integral to membrane; cytoplasmic vesicle; nucleus

Molecular Function: protein dimerization activity; protein binding; sterol response element binding; DNA binding; sequence-specific DNA binding; protein complex binding; chromatin binding; transcription factor activity; protein kinase binding

Biological Process: steroid metabolic process; cholesterol metabolic process; positive regulation of fatty acid biosynthetic process; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; lipid biosynthetic process; positive regulation of cholesterol biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; cellular response to starvation; negative regulation of insulin secretion; positive regulation of histone deacetylation; regulation of transcription, DNA-dependent; chemical signal regulation of heart rate; regulation of fatty acid metabolic process; response to glucose stimulus; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; lipid metabolic process; regulation of insulin secretion