AKR1C4 sirna

AKR1C4 siRNA (Human)

Cat. Num. AAA8201463

• Gene Names: AKR1C4; C11; CDR; DD4; CHDR; DD-4; HAKRA; 3-alpha-HSD
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AKR1C4; AKR1C4 siRNA (Human); CHDR; Aldo-keto reductase family 1 member C4; 3-alpha-HSD1; 3-alpha-hydroxysteroid dehydrogenase type I; Chlordecone reductase; CDR; Dihydrodiol dehydrogenase 4; DD-4; DD4; HAKRA; AKR1C4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AKR1C4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 323

• Applicable Applications for AKR1C4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AKR1C4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AKR1C4 sirna

siRNA to inhibit AKR1C4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 325652083
• NCBI GeneID: 1109
• NCBI Accession #: NP_001809.3
• NCBI GenBank Nucleotide #: NM_001818.3
• UniProt Accession #: P17516; Q5T6A3; Q8WW84; Q9NS54; AAB47003
• Molecular Weight: 37,067 Da
• NCBI Official Full Name: aldo-keto reductase family 1 member C4
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member C4
• NCBI Official Symbol: AKR1C4
• NCBI Official Synonym Symbols: C11; CDR; DD4; CHDR; DD-4; HAKRA; 3-alpha-HSD
• NCBI Protein Information: aldo-keto reductase family 1 member C4
• UniProt Protein Name: Aldo-keto reductase family 1 member C4
• Protein Family: Aldo-keto reductase family
• UniProt Gene Name: AKR1C4
• UniProt Synonym Gene Names: CHDR; CDR; DD-4; DD4
• UniProt Entry Name: AK1C4_HUMAN

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]

Uniprot Description

AKR1C4: Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha- androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20- alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route. Defects in AKR1C4 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. AKR1C4 mutations may act as modifier of disease severity in SRXY8 patients. A splicing mutation resulting in loss of exon 2 has been found in affected individuals also carrying mutation Val-79 in AKR1C2 (PubMed:21802064). Belongs to the aldo/keto reductase family.

Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Lipid Metabolism - C21-steroid hormone; EC 1.1.1.357; EC 1.1.1.225; Lipid Metabolism - primary bile acid biosynthesis; Lipid Metabolism - androgen and estrogen; Oxidoreductase

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: cytoplasm; cytosol

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; electron carrier activity; aldo-keto reductase activity; chlordecone reductase activity; bile acid transmembrane transporter activity; retinal dehydrogenase activity; 3(or 17)-alpha-hydroxysteroid dehydrogenase activity

Biological Process: bile acid and bile salt transport; steroid metabolic process; bile acid biosynthetic process; phototransduction, visible light; bile acid metabolic process; androgen metabolic process; retinoid metabolic process

Disease: 46,xy Sex Reversal 8