EPHA2 sirna

EPHA2 siRNA (Mouse)

Cat. Num. AAA8201090

• Gene Names: Epha2; Eck; Myk2; Sek2; Sek-2; AW545284
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EPHA2; EPHA2 siRNA (Mouse); ECK; MYK2; SEK2; Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; Tyrosine-protein kinase receptor MPK-5; Tyrosine-protein kinase receptor SEK-2; EPHA2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EPHA2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 977

• Applicable Applications for EPHA2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EPHA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EPHA2 sirna

siRNA to inhibit EPHA2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 32484983
• NCBI GeneID: 13836
• NCBI Accession #: NP_034269.2
• NCBI GenBank Nucleotide #: NM_010139.3
• UniProt Accession #: Q03145; Q3UNI2; Q60633; Q62212
• Molecular Weight: 108,852 Da
• NCBI Official Full Name: ephrin type-A receptor 2
• NCBI Official Synonym Full Names: Eph receptor A2
• NCBI Official Symbol: Epha2
• NCBI Official Synonym Symbols: Eck; Myk2; Sek2; Sek-2; AW545284
• NCBI Protein Information: ephrin type-A receptor 2
• UniProt Protein Name: Ephrin type-A receptor 2
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: Epha2
• UniProt Synonym Gene Names: Eck; Myk2; Sek2
• UniProt Entry Name: EPHA2_MOUSE

Uniprot Description

EphA2: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 AND -A5. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Overexpressed in many cancers including aggressive ovarian, cervical and breast carcinomas, and lung cancer. Expression correlates with degree of angiogenesis, metastasis and xenograft tumor growth. Soluble receptor inhibits tumor growth and angiogenesis in mice.

Protein type: Protein kinase, tyrosine (receptor); EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; TK group; Eph family

Cellular Component: cell projection; focal adhesion; cell surface; membrane; integral to plasma membrane; plasma membrane; integral to membrane; intracellular; cell junction

Molecular Function: transferase activity; protein binding; protein-tyrosine kinase activity; ephrin receptor activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: neural tube development; peptidyl-tyrosine phosphorylation; apoptosis; notochord formation; osteoclast differentiation; bone remodeling; regulation of blood vessel endothelial cell migration; protein amino acid phosphorylation; neuron differentiation; notochord morphogenesis; regulation of cell adhesion mediated by integrin; ephrin receptor signaling pathway; protein kinase B signaling cascade; angiogenesis; cell adhesion; cell differentiation; vasculogenesis; skeletal development; axial mesoderm formation; blood vessel development; cell migration; negative regulation of protein kinase B signaling cascade; mammary gland epithelial cell proliferation; regulation of angiogenesis; keratinocyte differentiation; osteoblast differentiation; DNA damage response, signal transduction resulting in induction of apoptosis; notochord cell development; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway