Rabbit anti-Mouse Epha2 Polyclonal Antibody | anti-Epha2 antibody

Epha2, ID (Epha2, Eck, Myk2, Sek2, Ephrin type-A receptor 2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, Tyrosine-protein kinase receptor MPK-5, Tyrosine-protein kinase receptor SEK-2) (APC)

Cat. Num. AAA6295975

• Gene Names: Epha2; Eck; Myk2; Sek2; Sek-2; AW545284
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Epha2; Polyclonal Antibody; ID (Epha2; Eck; Myk2; Sek2; Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; Tyrosine-protein kinase receptor MPK-5; Tyrosine-protein kinase receptor SEK-2) (APC); Tyrosine-protein kinase receptor SEK-2; anti-Epha2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Epha2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Epha2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-323 amino acids from the Central region of Mouse Epha2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Epha2 Antibody (Center) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the Epha2 antibody detected the Epha2 protein (arrow).)

Related Product Information for anti-Epha2 antibody

Receptor for members of the ephrin-A family. Binds to ephrin-A1,-A3,-A4 and-A5. Induces apoptosis in a TP53/p53-independent, caspase-8-dependent manner (By similarity). Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. May function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. May be involved in cell-cell interactions guiding early hindbrain development.

Product Categories/Family for anti-Epha2 antibody

Antibodies; Protein Kinases

References

Islam, S., et al. Dig. Dis. Sci. 55(9):2478-2488(2010). Kim, J., et al. Mol. Cell. Biol. 30(7):1582-1592(2010). Zirzow, S., et al. Dev. Biol. 336(2):145-155(2009). Jun, G., et al. PLoS Genet. 5 (7), E1000584 (2009): North, H.A., et al. Development 136(14):2467-2476(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 32484983
• NCBI GeneID: 13836
• NCBI Accession #: NP_034269.2
• NCBI GenBank Nucleotide #: NM_010139.3
• UniProt Accession #: Q03145; Q3UNI2; Q60633; Q62212
• Molecular Weight: 108,852 Da
• NCBI Official Full Name: ephrin type-A receptor 2
• NCBI Official Synonym Full Names: Eph receptor A2
• NCBI Official Symbol: Epha2
• NCBI Official Synonym Symbols: Eck; Myk2; Sek2; Sek-2; AW545284
• NCBI Protein Information: ephrin type-A receptor 2; epithelial cell kinase; tyrosine-protein kinase receptor ECK; tyrosine-protein kinase receptor MPK-5; tyrosine-protein kinase receptor SEK-2
• UniProt Protein Name: Ephrin type-A receptor 2
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: Epha2
• UniProt Synonym Gene Names: Eck; Myk2; Sek2
• UniProt Entry Name: EPHA2_MOUSE

Uniprot Description

Function: Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis. Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.17 Ref.18

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Ref.1

Subunit structure: Homodimer. Interacts with INPPL1; regulates activated EPHA2 endocytosis and degradation. Interacts (inactivated form) with PTK2/FAK1 and interacts (EFNA1 ligand-activated form) with PTPN11; regulates integrin-mediated adhesion. Interacts with ARHGEF16, DOCK4 and ELMO2; mediates ligand-independent activation of RAC1 which stimulates cell migration. Interacts with CLDN4; phosphorylates CLDN4 and may regulate tight junctions. Interacts with ACP1

By similarity. Interacts with SLA. Interacts (phosphorylated form) with VAV2, VAV3 and PI3-kinase p85 subunit (PIK3R1, PIK3R2 or PIK3R3); critical for the EFNA1-induced activation of RAC1 which stimulates cell migration. Interacts with ANKS1A. Ref.9 Ref.13 Ref.14 Ref.21

Subcellular location: Cell membrane; Single-pass type I membrane protein

By similarity. Cell projection › ruffle membrane; Single-pass type I membrane protein

By similarity. Cell projection › lamellipodium membrane; Single-pass type I membrane protein

By similarity. Cell junction › focal adhesion

By similarity. Note: Present at regions of cell-cell contacts but also at the leading edge of migrating cells

By similarity.

Tissue specificity: Expressed in the lung, intestine and liver. Ref.10

Developmental stage: First detected in gastrulation stage embryos (6.5-7.5 dpc) in ectodermal cells adjacent to the distal region of the primitive streak. By the neural plate stage (approximately 7.5 dpc), EPHA2 expression becomes restricted to the extreme distal end or node of the primitive streak. After the beginning of somitogenesis (approximately 8.0 dpc), expression persists in the node as this structure regresses toward the caudal end of the embryo. In addition, beginning at the mid head fold stage (approximately 7.75 dpc), we observe that EPHA2 exhibits a dynamic and spatially restricted expression pattern in the prospective hindbrain region. EPHA2 transcripts are initially detected in a 5-cell wide strip of mesodermal cells underlying prospective rhombomere 4 (R4). Subsequently at the beginning of somitogenesis, expression is observed in prospective R4. At the 4-8-somite stage, EPHA2 transcripts are observed in R4, mesenchymal cells underlying R4, and surface ectoderm in the vicinity of the developing second branchial arch. By the 10-somite stage, expression in these cells is down-regulated. Additionally, at the 5-8-somite stage, EPHA2 transcripts are detected initially in the lateral mesenchyme immediately underlying the surface ectoderm adjacent to R5 and R6, and subsequently in surface ectoderm overlying the developing third branchial arch. Ref.1 Ref.2 Ref.7 Ref.10

Post-translational modification: Autophosphorylates. Phosphorylated at Ser-898 by PKB; serum-induced phosphorylation which targets EPHA2 to the cell leading edge and stimulates cell migration. Phosphorylation by PKB is inhibited by EFNA1-activated EPHA2 which regulates PKB activity via a reciprocal regulatory loop. Dephosphorylated by ACP1

By similarity. Phosphorylated on tyrosine upon binding and activation by EFNA1. Phosphorylated residues Tyr-589 and Tyr-595 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-736 and Tyr-931 are required for binding PI3-kinase p85 subunit (PIK3R1, PIK3R2 or PIK3R3). These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and cell migration

By similarity. Ref.14Ubiquitinated by CHIP/STUB1. Ubiquitination is regulated by the HSP90 chaperone and regulates the receptor stability and activity through proteasomal degradation. ANKS1A prevents ubiquitination and degradation. Ref.21

Disruption phenotype: Mice are viable, fertile but exhibit aberrant development of tail vertebra and susceptibility to carcinogenesis. Ref.10 Ref.11 Ref.12

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.Contains 1 Eph LBD (Eph ligand-binding) domain.Contains 2 fibronectin type-III domains.Contains 1 protein kinase domain.Contains 1 SAM (sterile alpha motif) domain.