DLG2 sirna

DLG2 siRNA (Mouse)

Cat. Num. AAA8200862

• Gene Names: Dlg2; Dlgh2; PSD93; Gm1197; A330103J02Rik; B230218P12Rik; B330007M19Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DLG2; DLG2 siRNA (Mouse); DLGH2; Disks large homolog 2; Channel-associated protein of synapse-110; Chapsyn-110; Postsynaptic density protein PSD-93; DLG2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DLG2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 334

• Applicable Applications for DLG2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DLG2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DLG2 sirna

siRNA to inhibit DLG2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 340007427
• NCBI GeneID: 23859
• NCBI Accession #: NP_001229976.1
• NCBI GenBank Nucleotide #: NM_001243047.1
• UniProt Accession #: Q91XM9; Q8BXK7; Q8BYG5; F8VQM7
• Molecular Weight: 95,055 Da
• NCBI Official Full Name: disks large homolog 2 isoform 3
• NCBI Official Synonym Full Names: discs, large homolog 2 (Drosophila)
• NCBI Official Symbol: Dlg2
• NCBI Official Synonym Symbols: Dlgh2; PSD93; Gm1197; A330103J02Rik; B230218P12Rik; B330007M19Rik
• NCBI Protein Information: disks large homolog 2
• UniProt Protein Name: Disks large homolog 2
• Protein Family: Disks large
• UniProt Gene Name: Dlg2
• UniProt Synonym Gene Names: Dlgh2; Chapsyn-110
• UniProt Entry Name: DLG2_MOUSE

Uniprot Description

PSD-93: Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses. Interacts through its PDZ domains with NETO1. Interacts with NOS1/nNOS through second PDZ domain. Interacts with KCNJ2/Kir2.1 (via C-terminus) through one of its PDZ domains. Interacts with FRMPD4 (via C-terminus). Interacts with LRFN1, LRFN2 and LRFN4. Interacts with FASLG. Belongs to the MAGUK family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: postsynaptic membrane; voltage-gated potassium channel complex; cell projection; membrane; cell soma; dendrite; cytoplasm; plasma membrane; ionotropic glutamate receptor complex; synapse; cell junction

Molecular Function: protein C-terminus binding; ionotropic glutamate receptor binding; guanylate kinase activity; protein binding; protein heterodimerization activity; protein phosphatase binding; kinase binding; PDZ domain binding

Biological Process: synaptic transmission; nervous system development; sensory perception of pain; neuronal ion channel clustering; receptor clustering; nucleotide phosphorylation