AXIN2 sirna

AXIN2 siRNA (Mouse)

Cat. Num. AAA8200861

• Gene Names: Axin2; Axi1; Axil; Conductin
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AXIN2; AXIN2 siRNA (Mouse); Axin-2; Axin-like protein; Axil; Axis inhibition protein 2; Conductin; AXIN2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AXIN2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 840

• Applicable Applications for AXIN2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AXIN2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AXIN2 sirna

siRNA to inhibit AXIN2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 40254370
• NCBI GeneID: 12006
• NCBI Accession #: NP_056547.3
• NCBI GenBank Nucleotide #: NM_015732.4
• UniProt Accession #: O88566; Q6PFZ9; Q9QXJ6
• Molecular Weight: 92,906 Da
• NCBI Official Full Name: axin-2
• NCBI Official Synonym Full Names: axin2
• NCBI Official Symbol: Axin2
• NCBI Official Synonym Symbols: Axi1; Axil; Conductin
• NCBI Protein Information: axin-2
• UniProt Protein Name: Axin-2
• Protein Family: Axin
• UniProt Gene Name: Axin2
• UniProt Synonym Gene Names: Axil
• UniProt Entry Name: AXIN2_MOUSE

Uniprot Description

axin 2: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Is downregulated during progression of esophageal squamous cell carcinoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.

Protein type: Adaptor/scaffold

Cellular Component: microtubule cytoskeleton; nucleoplasm; Golgi apparatus; centrosome; protein complex; cytoplasmic microtubule; postsynaptic density; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; cell cortex; beta-catenin destruction complex; nucleus

Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; beta-catenin binding; protein kinase binding; GTPase activator activity

Biological Process: cell death; intramembranous ossification; somitogenesis; Wnt receptor signaling pathway; mRNA stabilization; regulation of Wnt receptor signaling pathway; dorsal/ventral axis specification; maintenance of DNA repeat elements; bone mineralization; odontogenesis; negative regulation of cell proliferation; cell proliferation; regulation of transcription, DNA-dependent; regulation of mismatch repair; positive regulation of protein kinase activity; negative regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of GTPase activity