RTEL1 sirna

RTEL1 siRNA (Human)

Cat. Num. AAA8200840

• Gene Names: RTEL1; NHL; RTEL; DKCA4; DKCB5; PFBMFT3; C20orf41
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RTEL1; RTEL1 siRNA (Human); C20orf41; KIAA1088; NHL; Regulator of telomere elongation helicase 1; Novel helicase-like; RTEL1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RTEL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1300

• Applicable Applications for RTEL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RTEL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RTEL1 sirna

siRNA to inhibit RTEL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 545688900
• NCBI GeneID: 51750
• NCBI Accession #: NP_001269938.1
• NCBI GenBank Nucleotide #: NM_001283009.1
• UniProt Accession #: Q9NZ71; Q5JTV3; Q5JTV4; Q9BW37; Q9H402; A2A397; A2A398; B4DRM5; B4DYM3; B4E3N6; E1P5J4; E1P5J5
• Molecular Weight: 108,457 Da
• NCBI Official Full Name: regulator of telomere elongation helicase 1 isoform 3
• NCBI Official Synonym Full Names: regulator of telomere elongation helicase 1
• NCBI Official Symbol: RTEL1
• NCBI Official Synonym Symbols: NHL; RTEL; DKCA4; DKCB5; PFBMFT3; C20orf41
• NCBI Protein Information: regulator of telomere elongation helicase 1
• UniProt Protein Name: Regulator of telomere elongation helicase 1
• Protein Family: Regulator of telomere elongation helicase
• UniProt Gene Name: RTEL1
• UniProt Entry Name: RTEL1_HUMAN

NCBI Description

This gene encodes a DNA helicase which functions in the stability, protection and elongation of telomeres and interacts with proteins in the shelterin complex known to protect telomeres during DNA replication. Mutations in this gene have been associated with dyskeratosis congenita and Hoyerall-Hreidarsson syndrome. Read-through transcription of this gene into the neighboring downstream gene, which encodes tumor necrosis factor receptor superfamily, member 6b, generates a non-coding transcript. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2013]

Uniprot Description

RTEL1: ATP-dependent DNA helicase required to suppress inappropriate homologous recombination, thereby playing a central role DNA repair and in the maintenance of genomic stability. Antagonizes homologous recombination by promoting the disassembly of D loop recombination intermediates. Also required to regulate telomere length; probably due to its anti-recombinase function. Belongs to the helicase family. RAD3/XPD subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Helicase; EC 3.6.4.12; DNA repair, damage

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; ATP binding

Biological Process: DNA repair; telomere maintenance; DNA duplex unwinding

Disease: Dyskeratosis Congenita, Autosomal Recessive, 5; Pulmonary Fibrosis And/or Bone Marrow Failure, Telomere-related, 3