LDLRAP1 sirna

LDLRAP1 siRNA (Human)

Cat. Num. AAA8200800

• Gene Names: LDLRAP1; ARH; ARH1; ARH2; FHCB1; FHCB2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LDLRAP1; LDLRAP1 siRNA (Human); ARH; Low density lipoprotein receptor adapter protein 1; Autosomal recessive hypercholesterolemia protein; LDLRAP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LDLRAP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 308

• Applicable Applications for LDLRAP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LDLRAP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LDLRAP1 sirna

siRNA to inhibit LDLRAP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 132626790
• NCBI GeneID: 26119
• NCBI Accession #: NP_056442.2
• NCBI GenBank Nucleotide #: NM_015627.2
• UniProt Accession #: Q5SW96; Q6TQS9; Q8N2Y0; Q9UFI9; A2BHI5
• Molecular Weight: 33,885 Da
• NCBI Official Full Name: low density lipoprotein receptor adapter protein 1
• NCBI Official Synonym Full Names: low density lipoprotein receptor adaptor protein 1
• NCBI Official Symbol: LDLRAP1
• NCBI Official Synonym Symbols: ARH; ARH1; ARH2; FHCB1; FHCB2
• NCBI Protein Information: low density lipoprotein receptor adapter protein 1
• UniProt Protein Name: Low density lipoprotein receptor adapter protein 1
• Protein Family: Low density lipoprotein receptor adapter protein
• UniProt Gene Name: LDLRAP1
• UniProt Synonym Gene Names: ARH
• UniProt Entry Name: ARH_HUMAN

NCBI Description

The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia. [provided by RefSeq, Jul 2008]

Uniprot Description

LDLRAP1: Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. Defects in LDLRAP1 are the cause of autosomal recessive hypercholesterolemia (ARH). ARH is a disorder caused by defective internalization of LDL receptors (LDLR) in the liver. ARH has the clinical features of familial hypercholesterolemia (FH) homozygotes, including severely elevated plasma LDL cholesterol, tuberous and tendon xanthomata, and premature atherosclerosis. LDL receptor (LDLR) activity measured in skin fibroblasts is normal, as the LDL binding ability.

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: recycling endosome; internal side of plasma membrane; AP-1 adaptor complex; axon; early endosome; basal plasma membrane; neurofilament; AP-2 adaptor complex; cytosol

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; clathrin binding; protein binding; receptor signaling complex scaffold activity; low-density lipoprotein receptor binding; phosphotyrosine binding; beta-amyloid binding

Biological Process: cholesterol metabolic process; receptor-mediated endocytosis; cholesterol homeostasis; positive regulation of signal transduction; transport; amyloid precursor protein metabolic process; receptor internalization; regulation of protein binding; positive regulation of receptor-mediated endocytosis

Disease: Hypercholesterolemia, Autosomal Recessive