NEIL1 sirna

NEIL1 siRNA (Mouse)

Cat. Num. AAA8200497

• Gene Names: Neil1; Nei1; 2810450N13Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NEIL1; NEIL1 siRNA (Mouse); NEI1; Endonuclease 8-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; Endonuclease VIII-like 1; Nei homolog 1; NEH1; Nei-like protein 1; NEIL1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NEIL1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 389

• Applicable Applications for NEIL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NEIL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NEIL1 sirna

siRNA to inhibit NEIL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22094129
• NCBI GeneID: 72774
• NCBI Accession #: NP_082623.1
• NCBI GenBank Nucleotide #: NM_028347.2
• UniProt Accession #: Q8K4Q6; Q80V58; Q9CYT9
• Molecular Weight: 28,453 Da
• NCBI Official Full Name: endonuclease 8-like 1
• NCBI Official Synonym Full Names: nei endonuclease VIII-like 1 (E. coli)
• NCBI Official Symbol: Neil1
• NCBI Official Synonym Symbols: Nei1; 2810450N13Rik
• NCBI Protein Information: endonuclease 8-like 1
• UniProt Protein Name: Endonuclease 8-like 1
• Protein Family: Endonuclease
• UniProt Gene Name: Neil1
• UniProt Synonym Gene Names: Nei1; NEH1
• UniProt Entry Name: NEIL1_MOUSE

Uniprot Description

NEIL1: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8- oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Belongs to the FPG family.

Protein type: Hydrolase; Lyase; DNA repair, damage; EC 4.2.99.18

Cellular Component: nucleoplasm; cytoskeleton; cytoplasm; chromosome; nucleus

Molecular Function: single-strand selective uracil DNA N-glycosylase activity; protein C-terminus binding; hydrolase activity, hydrolyzing N-glycosyl compounds; uracil DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) lyase activity; zinc ion binding; hydrolase activity; DNA N-glycosylase activity; lyase activity; hydrolase activity, acting on glycosyl bonds; purine-specific mismatch base pair DNA N-glycosylase activity; DNA-3-methyladenine glycosylase III activity; DNA binding; nucleic acid binding; damaged DNA binding; catalytic activity

Biological Process: metabolic process; base-excision repair; nucleotide-excision repair; response to oxidative stress; DNA repair; response to DNA damage stimulus; negative regulation of nuclease activity