DRD1 sirna

DRD1 siRNA (Rat)

Cat. Num. AAA8200043

• Gene Names: Drd1; D1a; Drd-1; Drd1a
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DRD1; DRD1 siRNA (Rat); DRD1A; D(1A) dopamine receptor; Dopamine D1 receptor; DRD1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: DRD1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 446

• Applicable Applications for DRD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat DRD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DRD1 sirna

siRNA to inhibit DRD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 118229
• NCBI GeneID: 24316
• UniProt Accession #: P18901; P21669
• Molecular Weight: 49,428 Da
• NCBI Official Full Name: D(1A) dopamine receptor
• NCBI Official Synonym Full Names: dopamine receptor D1
• NCBI Official Symbol: Drd1
• NCBI Official Synonym Symbols: D1a; Drd-1; Drd1a
• NCBI Protein Information: D(1A) dopamine receptor
• UniProt Protein Name: D(1A) dopamine receptor
• Protein Family: D(1A) dopamine receptor
• UniProt Gene Name: Drd1
• UniProt Synonym Gene Names: Drd1a
• UniProt Entry Name: DRD1_RAT

NCBI Description

induces dopamine sensitive adenylate cyclase activation; plays a role in regulating food intake [RGD, Feb 2006]

Uniprot Description

DRD1: a G-protein coupled receptor.One of the five types (D1 to D5) of receptors for dopamine. The most abundant dopamine receptor in the central nervous system. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Interacts with calcyon.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Cellular Component: endoplasmic reticulum membrane; cell; integral to plasma membrane; endoplasmic reticulum; dendrite; dendritic spine; integral to membrane; caveola; cell soma; membrane; axon; plasma membrane; dendritic shaft; intracellular; nerve terminal; nucleus

Molecular Function: angiotensin receptor binding; G-protein alpha-subunit binding; drug binding; protein phosphatase binding; ATPase binding; G-protein coupled receptor activity; protein binding; dopamine receptor activity; protein heterodimerization activity; dopamine binding; protein complex binding; dopamine D1 receptor-like receptor activity; D3 dopamine receptor binding; receptor binding

Biological Process: response to nicotine; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); prepulse inhibition; thermoregulation; G-protein signaling, adenylate cyclase activating pathway; adult walking behavior; response to organic cyclic substance; protein import into nucleus; conditioned taste aversion; positive regulation of cAMP biosynthetic process; phosphatidylinositol metabolic process; positive regulation of adenylate cyclase activity; phosphatidylinositol catabolic process; response to food; response to drug; dopamine transport; dentate gyrus development; cerebral cortex GABAergic interneuron migration; adenylate cyclase activation; mating behavior; transmission of nerve impulse; social behavior; response to amino acid stimulus; response to organic nitrogen; G-protein signaling, coupled to cyclic nucleotide second messenger; response to ethanol; cellular response to insulin stimulus; response to steroid hormone stimulus; glucose import; regulation of vasoconstriction; operant conditioning; dopamine receptor signaling pathway; negative regulation of protein kinase activity; response to activity; positive regulation of membrane potential; regulation of long-term neuronal synaptic plasticity; regulation of ion transport; synaptic transmission, dopaminergic; peristalsis; startle response; behavioral fear response; generation of action potential; negative regulation of circadian sleep/wake cycle, sleep; response to morphine; locomotory behavior; orbitofrontal cortex development; positive regulation of synaptic transmission, glutamatergic; vasodilation; response to estradiol stimulus; intracellular protein transport; synaptic transmission; synaptogenesis; muscle contraction; behavioral response to cocaine; visual learning; feeding behavior; negative regulation of cell migration; associative learning; dopamine receptor, adenylate cyclase activating pathway; grooming behavior; response to retinoic acid; striatum development; maternal behavior; hippocampus development; regulation of dopamine metabolic process; response to amphetamine; calcium-mediated signaling; learning; response to cocaine; astrocyte development; memory; G-protein coupled receptor protein signaling pathway; habituation; sensitization; dopamine receptor, phospholipase C activating pathway; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of cell migration