Betacellulin Active Protein | BTC active protein

Betacellulin Protein, Cynomolgus, Recombinant (hFc Tag)

Cat. Num. AAA8123529

• Purity: >85% as determined by SDS-PAGE
• Synonyms: Betacellulin; Betacellulin Protein; Cynomolgus; Recombinant (hFc Tag); Cynomolgus BTC/Betacellulin Protein (Fc Tag); betacellulin; BTC active protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Tyr111

• Species: Cynomolgus
• Activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 5-40ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asp 32
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the cynomolgus BTC (XP_001101880.1)(Met1-Tyr111) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for BTC active protein

Background: Betacellulin(BTC) is a member of the epidermal growth factor (EGF) family. These soluble proteins are ligands for one or more of the four receptor tyrosine kinases encoded by the ErbB gene family (ErbB-1/epidermal growth factor receptor (EGFR), neu/ErbB-2/HER2, ErbB-3/HER3 and ErbB-4/HER4). Betacellulin is a 32-kilodalton glycoprotein that appears to be processed from a larger transmembrane precursor by proteolytic cleavage. This protein is a ligand for the EGF receptor. BTC is a polymer of about 62-111 amino acid residues. Secondary Structure: 6% helical (1 helices; 3 residues)36% beta sheet (5 strands; 18 residues). BTC was originally identified as a growth-promoting factor in mouse pancreatic beta-cell carcinoma cell line and has since been identified in humans. It plays a role in the growth and development of the neonate and/or mammary gland function. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

References

Shing Y, et al. (1993) Betacellulin: a mitogen from pancreatic beta cell tumors. Science. 259(5101): 1604-7.Riese DJ, et al. (1996) Betacellulin activates the epidermal growth factor receptor and erbB-4, and induces cellular response patterns distinct from those stimulated by epidermal growth factor or neuregulin-beta. Oncogene. 12(2): 345-53.Bastian SE, et al. (2001) Measurement of betacellulin levels in bovine serum, colostrum and milk. J Endocrinol. 168: 203-12.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant cynomolgus BTC is a disulfide-linked homodimer. The reduced monomer comprises 321 amino acids and has a calculated molecular mass of 36kDa.The apparent molecular mass of the protein is approximately 46kDa respectively in SDS-PAGE.
• Protein Family: Probetacellulin