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Lp-PLA2/PLA2G7 Recombinant Protein | PLA2G7 recombinant protein
Lp-PLA2/PLA2G7 Protein, Mouse, Recombinant (His Tag)
Gene Names
Pla2g7; PAF-AH; R75400
Purity
>90% as determined by SDS-PAGE
Synonyms
Lp-PLA2/PLA2G7; Lp-PLA2/PLA2G7 Protein; Mouse; Recombinant (His Tag); Mouse PLA2G7/PAFAH Protein (His Tag); R75400 Protein; phospholipase A2 group VII; PLA2G7 recombinant protein
Host
HEK293 Cells
Purity/Purification
>90% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Asn440
Species
Mouse
Endotoxin
<1.0EU per ug protein as determined by the LAL method.
Predicted N Terminal
Phe 22
Tag
C-His
Protein Construction
A DNA sequence encoding the mouse Pla2g7 (NP_038765.2)(Met1-Asn440) was expressed with a polyhistidine tag at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for PLA2G7 recombinant protein
Background: Platelet-activating factor acetylhydrolase, also known as 1-alkyl-2-acetylglycerophosphocholine esterase, 2-acetyl-1-alkylglycero-phosphocholine esterase, Group-VIIA phospholipase A2, LDL-associated phospholipase A2, PAF 2-acylhydrolase, PLA2G7 and PAFAH, is a secreted protein that belongs to the AB hydrolase superfamily and Lipase family. PLA2G7/PAFAH modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. It has specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. PLA2G7/PAFAH is a potent pro- and anti-inflammatory molecule that has been implicated in multiple inflammatory disease processes, including cardiovascular disease. PLA2G7 also represents an important, potentially functional candidate in the pathophysiology of coronary artery disease (CAD). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PLA2G7 deficiency). It is a trait that is present in 27% of Japanese. It could have a significant physiologic effect in the presence of inflammatory bodily responses.
References
Stafforini D.M., et al., 1996, J. Clin. Invest. 97:2784-2791. Yoshida H., et al., 1998, Thromb. Haemost. 80:372-375. Yamada Y., et al., 1998, Metabolism 47:177-181. Kruse S., et al., 2000, Am. J. Hum. Genet. 66:1522-1530.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,258 Da
NCBI Official Full Name
platelet-activating factor acetylhydrolase
NCBI Official Synonym Full Names
phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
NCBI Official Symbol
Pla2g7
NCBI Official Synonym Symbols
PAF-AH; R75400
NCBI Protein Information
platelet-activating factor acetylhydrolase
UniProt Protein Name
Platelet-activating factor acetylhydrolase
Protein Family
UniProt Gene Name
Pla2g7
UniProt Synonym Gene Names
Pafah; PAF acetylhydrolase; LDL-PLA(2)
Uniprot Description
Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.
Research Articles on PLA2G7
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Product Notes
The PLA2G7 pla2g7 (Catalog #AAA8123221) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Asn44 0. It is sometimes possible for the material contained within the vial of "Lp-PLA2/PLA2G7, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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