Pleiotrophin/PTN Active Protein | PTN active protein

Pleiotrophin/PTN Protein, Mouse, Recombinant

Cat. Num. AAA8123086

• Gene Names: Ptn; OSF; HARP; HBBN; HBNF; Osf1; Osf-1; HB-GAM; HBGF-8
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Pleiotrophin/PTN; Pleiotrophin/PTN Protein; Mouse; Recombinant; Mouse Pleiotrophin/PTN/HB-GAM Protein; HARP Protein; HB-GAM Protein; HBBN Protein; HBGF-8 Protein; HBNF Protein; OSF Protein; Osf-1 Protein; Osf1 Protein; pleiotrophin; PTN active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 1M NaCl, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp168

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 33
• Tag: Native
• Protein Construction: A DNA sequence encoding the mouse PTN (P63089)(Met1-Asp168) was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse PTN at 10ug/ml (100uL/well) can bind rat SDC1-Fc. The EC50 of rat SDC1-Fc is 0.4-1.1ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized mouse PTN at 10 ug/ml (100 ul/well) can bind rat SDC1-Fc, The EC50 of rat SDC1-Fc is 0.4-1.1 ug/ml.)

Related Product Information for PTN active protein

Background: HB-GAM belongs to the pleiotrophin family. During embryonic and early postnatal development, HB-GAM is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. While in the adult central nervous system, it is expressed in an activity-dependent manner in the hippocampus where it can suppress long term potentiation induction. HB-GAM has a low expression in other areas of the adult brain, but it can be induced by ischemic insults, or targeted neuronal damage in the entorhinal cortex or in the substantia nigra pars compacta. It is structurally related to midkine and retinoic acid induced heparin-binding protein and has a high affinity for heparin. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also functions as a secreted growth factor and induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells.

References

Vanderwinden JM, et al. (1992) Cellular distribution of the new growth factor pleiotrophin (HB-GAM) mRNA in developing and adult rat tissues. Anat Embryol. 186(4):387-406.Lauri SE, et al. (1996) Activity-induced enhancement of HB-GAM expression in rat hippocampal slices. Neuroreport. 7(10):1670-4.Pavlov I, et al. (2002) Role of heparin-binding growth-associated molecule (HB-GAM) in hippocampal LTP and spatial learning revealed by studies on overexpressing and knockout mice. Mol Cell Neurosci. 20(2):330-42.

NCBI and Uniprot Product Information

• NCBI GI #: 6679543
• NCBI GeneID: 19242
• NCBI Accession #: NP_032999.1
• NCBI GenBank Nucleotide #: NM_008973.2
• UniProt Accession #: P63089; P20935
• Molecular Weight: 18,869 Da
• NCBI Official Full Name: pleiotrophin
• NCBI Official Synonym Full Names: pleiotrophin
• NCBI Official Symbol: Ptn
• NCBI Official Synonym Symbols: OSF; HARP; HBBN; HBNF; Osf1; Osf-1; HB-GAM; HBGF-8
• NCBI Protein Information: pleiotrophin
• UniProt Protein Name: Pleiotrophin
• Protein Family: Pleiotrophin
• UniProt Gene Name: Ptn
• UniProt Synonym Gene Names: PTN; HBBM; HBGF-8; HB-GAM; HBNF; OSF-1

Uniprot Description

Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. Binds to cell-surface target proteins via their chondroitin sulfate groups. Down-regulates PTPRZ1 activity.