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Factor D Recombinant Protein | CFD recombinant protein
Factor D Protein, Mouse, Recombinant (His Tag)
Gene Names
Cfd; DF; Adn
Purity
>98% as determined by SDS-PAGE
Synonyms
Factor D; Factor D Protein; Mouse; Recombinant (His Tag); Mouse Adipsin/Complement Factor D/CFD Protein (His Tag); Adn Protein; DF Protein; complement factor D (adipsin); CFD recombinant protein
Host
HEK293 Cells
Purity/Purification
>98% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Ser258
Species
Mouse
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Gln 20
Tag
C-His
Protein Construction
A DNA sequence encoding the full length of mouse CFD isoform 2 (P03953-2)(Met 1-Ser 258) was expressed, with a polyhistidine tag at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for CFD recombinant protein
Background: Complement factor D, also known as Adipsin, C3 convertase activator, Properdin factor D and CFD is a secreted protein which belongs to thepeptidase S1 family. CFD/Adipsin contains onepeptidase S1 domain. Complement factor D (CFD/Adipsin) is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. Complement factor D (CFD/Adipsin) has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Complement factor D (CFD/Adipsin) cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Complement factor D (CFD/Adipsin) is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Defects in CFD/Adipsin are the cause of complement factor D deficiency (CFD deficiency) which predisposes to invasive meningococcal disease.
Product Categories/Family for CFD recombinant protein
References
Volanakis JE, et al.,1996, Protein Sci. 5 (4): 553-64. Searfoss,G.H et al., 2003, J Biol Chem. 278 (46):46107-16. Ukkola,O. et al., 2003, Eur J Clin Nutr. 57 (9):1073-8. Ronti T, et al., 2006, Clin. Endocrinol. (Oxf) 64 (4): 355-65.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,986 Da
NCBI Official Full Name
complement factor D isoform 2
NCBI Official Synonym Full Names
complement factor D (adipsin)
NCBI Official Symbol
Cfd
NCBI Official Synonym Symbols
DF; Adn
NCBI Protein Information
complement factor D
UniProt Protein Name
Complement factor D
Protein Family
UniProt Gene Name
Cfd
UniProt Synonym Gene Names
Adn; Df
UniProt Entry Name
CFAD_MOUSE
Uniprot Description
CFD: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Defects in CFD are the cause of complement factor D deficiency (CFDD). CFDD is an immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Belongs to the peptidase S1 family.
Protein type: Protease; Secreted, signal peptide; EC 3.4.21.46; Secreted
Cellular Component: extracellular region; extracellular space
Molecular Function: endopeptidase activity; hydrolase activity; peptidase activity; serine-type endopeptidase activity; serine-type peptidase activity
Biological Process: complement activation, alternative pathway; immune system process; innate immune response; Notch signaling pathway; proteolysis
Research Articles on CFD
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Product Notes
The CFD cfd (Catalog #AAA8122807) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Ser25 8. It is sometimes possible for the material contained within the vial of "Factor D, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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