Prolactin Active Protein | PRL active protein

Prolactin Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122707

• Purity: >92% as determined by SDS-PAGE
• Synonyms: Prolactin; Prolactin Protein; Mouse; Recombinant (His Tag); Mouse PRL/Prolactin Protein (His Tag); AV290867 Protein; Prl1a1 Protein; prolactin; PRL active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Leu32-Cys228

• Species: Mouse
• Activity: Measured by its ability to promote proliferation of INS-1 cells. The ED50 for this effect is typically 50-500ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse PRL (NP_035294.2)(Leu 32-Cys 228) was expressed, with an initial Met at the N-terminus and a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to promote proliferation of INS-1 cells. The ED50 for this effect is typically 50-500 ng/mL.)

Related Product Information for PRL active protein

Background: Prolactin (PRL) is a hormone with multiple actions in the central nervous system (CNS) spanning from physiology to pathology. PRL exerts different actions through its receptors that can be found in both neurons and glial cells (astrocytes, microglia and oligodendrocytes) of the brain. It is generally believed that in vertebrates, prolactin (PRL) is predominantly synthesized and released by pituitary lactotrophs and plays important roles in many physiological processes via activation of PRL receptor (PRLR), including water and electrolyte balance, reproduction, growth and development, metabolism, immuno-modulation, and behavior.

References

Jara LJ, et al. (2011) Prolactin and autoimmunity. Clin Rev Allergy Immunol. 40(1): 50-9.Urban A, et al. (2007) Prolactin as a factor for increased platelet aggregation. Neuro Endocrinol Lett. 28(4): 518-23.Charoenphandhu N, et al. (2007) Prolactin is an important regulator of intestinal calcium transport. Can J Physiol Pharmacol. 85(6): 569-81.Tworoger SS, et al. (2006) Prolactin and breast cancer risk. Cancer Lett. 243(2): 160-9.Ben-Jonathan N, et al. (2006) Focus on prolactin as a metabolic hormone. Trends Endocrinol Metab. 17(3): 110-6.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant mouse PRL consists of 207 amino acids and has a calculated molecular mass of 23.8kDa. It migrates as an approximately 26kDa band in SDS-PAGE under reducing conditions.
• Protein Family: Prolactin