Factor IX Recombinant Protein | F9 recombinant protein

Factor IX Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122705

• Gene Names: F9; Cf9; Cf-9; AW111646
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Factor IX; Factor IX Protein; Mouse; Recombinant (His Tag); Mouse Coagulation Factor IX/FIX/F9 Protein (His Tag); AW111646 Protein; Cf-9 Protein; Cf9 Protein; coagulation factor IX; F9 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr471

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 29
• Tag: C-His
• Protein Construction: A DNA sequence encoding the full length of mouse F9 (P16294)(Met 1-Thr 471) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for F9 recombinant protein

Background: Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX/F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and onepeptidase S1 domain. Coagulation factor IX/F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca²⁺ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX/F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX/F9 are also the cause of recessive X-linked hemophilia B (HEMB) which also known as Christmas disease.

References

Onay U.V., et al., 2003, Br. J. Haematol. 120:656-659. Vidal F., et al., 2000, Br. J. Haematol. 111:549-551. Simioni P., et al., 2009, N. Engl. J. Med. 361:1671-1675. Espinos C., et al., 2009, Haematologica 88:235-236.

NCBI and Uniprot Product Information

• NCBI GI #: 73661202
• NCBI GeneID: 14071
• NCBI Accession #: NP_032005.1
• NCBI GenBank Nucleotide #: NM_007979.1
• UniProt Accession #: P16294; Q3UES1
• Molecular Weight: 160,000
• NCBI Official Full Name: coagulation factor IX
• NCBI Official Synonym Full Names: coagulation factor IX
• NCBI Official Symbol: F9
• NCBI Official Synonym Symbols: Cf9; Cf-9; AW111646
• NCBI Protein Information: coagulation factor IX; christmas factor
• UniProt Protein Name: Coagulation factor IX
• Protein Family: Coagulation factor
• UniProt Gene Name: F9
• UniProt Synonym Gene Names: Cf9
• UniProt Entry Name: FA9_MOUSE

NCBI Description

This gene encodes a vitamin K-dependent serine protease that plays a critical role in the intrinsic pathway of blood coagulation. The encoded protein is an inactive zymogen that is activated by coagulation factor XIa to generate factor IXa, a heterodimer containing heavy and light chains. In association with factor VIII, membrane phospholipids and calcium ions, factor IXa cleaves the inactive zymogen factor X to generate active factor Xa. Genetic deletion of this gene in mice results in a severe bleeding phenotype. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

F9: Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. Defects in F9 are the cause of recessive X-linked hemophilia B (HEMB); also known as Christmas disease. Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide. Defects in F9 are the cause of thrombophilia due to factor IX defect (THPH8). A hemostatic disorder characterized by a tendency to thrombosis. Belongs to the peptidase S1 family.

Protein type: Secreted; Secreted, signal peptide; EC 3.4.21.22; Protease

Cellular Component: extracellular region

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; calcium ion binding; catalytic activity

Biological Process: hemostasis; proteolysis; blood coagulation