VEGFD active protein

VEGFD Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122569

• Gene Names: Vegfd; Figf; VEGF-D; AI325264
• Purity: >95% as determined by SDS-PAGE
• Synonyms: VEGFD; VEGFD Protein; Mouse; Recombinant (hFc Tag); Mouse VEGF-D/VEGFD/FIGF Protein (Fc Tag); AI325264 Protein; VEGF-D Protein; Vegfd Protein; c-fos induced growth factor (vascular endothelial growth factor D); VEGFD active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Phe98-Ser206

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mature form of mouse FIGF (P97946)(Phe 98-Ser 206) was fused with the Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized human VEGFR3-His at 10ug/mL (100uL/well) can bind mouse Fc-VEGFD. The EC50 of mouse Fc-VEGFD is 49ng/mL.; 3. Immobilized mouse VEGFR3-His at 10ug/mL (100uL/well) can bind mouse Fc-VEGFD. The EC50 of mouse Fc-VEGFD is 44ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human VEGFR3-His at 10 ug/mL (100 ul/well) can bind mouse Fc-VEGFD, The EC50 of mouse Fc-VEGFD is 49 ng/mL. 3. Immobilized mouse VEGFR3-His at 10 ug/mL (100 ul/well) can bind mouse Fc-VEGFD, The EC50 of mouse Fc-VEGFD is 44 ng/mL.)

Related Product Information for VEGFD active protein

Background: Vascular endothelial growth factor D (VEGF-D), also known as C-fos induced growth factor (FIGF), belongs to the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. FIGF protein is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. FIGF protein is secreted as a non-covelent homodimer in an antiparallel fashion. Human FIGF protein is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin. FIGF protein is structurally and functionally similar to VEGF-C. Therefore, FIGF protein binds and activates VEGFR-2 (Flk1) and VEGFR-3 (Flt4) receptors, and may particularly be involved in cancers, such as breast cancer, epithelial ovarian carcinoma and so on.

References

Avantaggiato V, et al. (1998) Embryonic expression pattern of the murine figf gene, a growth factor belonging to platelet-derived growth factor/vascular endothelial growth factor family. Mech Dev. 73(2):221-4.Rocchigiani M, et al. (1998) Human FIGF: cloning, gene structure, and mapping to chromosome Xp22.1 between the PIGA and the GRPR genes. Genomics 47(2):207-16.Karpanen T, et al. (2008) VEGF-D: a modifier of embryonic lymphangiogenesis. Blood. 112(5): 1547-8.

NCBI and Uniprot Product Information

• NCBI GI #: 819231786
• NCBI GeneID: 14205
• NCBI Accession #: NP_001295418.1
• NCBI GenBank Nucleotide #: NM_001308489.1
• UniProt Accession #: P97946; A2AIH3
• Molecular Weight: 40,909 Da
• NCBI Official Full Name: vascular endothelial growth factor D isoform VEGF-D326 preproprotein
• NCBI Official Synonym Full Names: vascular endothelial growth factor D
• NCBI Official Symbol: Vegfd
• NCBI Official Synonym Symbols: Figf; VEGF-D; AI325264
• NCBI Protein Information: vascular endothelial growth factor D
• UniProt Protein Name: Vascular endothelial growth factor D
• Protein Family: Vascular endothelial growth factor
• UniProt Gene Name: Vegfd
• UniProt Synonym Gene Names: VEGF-D; FIGF

NCBI Description

This gene encodes a member of the vascular endothelial growth factor (VEGF) family that acts as a ligand for vascular endothelial growth factor receptor 3. Signalling through this protein is important for growth and development of endothelial and vascular tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]

Uniprot Description

Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-3 (Flt4) receptor.