carbonic anhydrase XII Active Protein | CA12 active protein

carbonic anhydrase XII Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122460

• Gene Names: Car12; Ca12; CA-XII; AI314958; 2310047E01Rik
• Purity: >95% as determined by SDS-PAGE
• Synonyms: carbonic anhydrase XII; carbonic anhydrase XII Protein; Mouse; Recombinant (His Tag); Mouse Carbonic Anhydrase XII/Car12 Protein (His Tag); 2310047E01Rik Protein; AI314958 Protein; Car12 Protein; CA12 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser301

• Species: Mouse
• Activity: Measured by its esterase activity. The specific activity is >50pmoles/min/ug, as measured with 1mM 4-Nitrophenyl acetate and 0.4 ug enzyme at 400nM in 100 uL of 12.5mM Tris, 75mM NaCl, pH7.5.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Ser 301) of mouse CA12 (NP_848483.2) precursor was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CA12 active protein

Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning alpha-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.

References

Sahin, U. et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 92 (25): 11810-11813. Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596 - 12601. Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 - 16903. Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262. Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602. Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.

NCBI and Uniprot Product Information

• NCBI GI #: 31541884
• NCBI GeneID: 76459
• NCBI Accession #: NP_848483.2
• NCBI GenBank Nucleotide #: NM_178396.3
• Molecular Weight: 39,695 Da
• NCBI Official Full Name: carbonic anhydrase XII
• NCBI Official Synonym Full Names: carbonic anhydrase 12
• NCBI Official Symbol: Car12
• NCBI Official Synonym Symbols: Ca12; CA-XII; AI314958; 2310047E01Rik
• NCBI Protein Information: carbonic anhydrase 12
• Protein Family: Carbonic anhydrase

NCBI Description

This gene encodes a membrane-bound member of the alpha carbonic anhydrase family of enzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. These proteins participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]