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GADD45G recombinant protein
GADD45G Protein, Human, Recombinant
Gene Names
GADD45G; CR6; DDIT2; GRP17; GADD45gamma
Purity
>95% as determined by SDS-PAGE
Synonyms
GADD45G; GADD45G Protein; Human; Recombinant; Human GADD45G/CR6 Protein; CR6 Protein; DDIT2 Protein; GADD45gamma Protein; GRP17 Protein; growth arrest and DNA-damage-inducible; gamma; GADD45G recombinant protein
Host
E Coli
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Glu159
Species
Human
Predicted N Terminal
Met
Tag
Native
Protein Construction
A DNA sequence encoding human GADD45G (O95257)(Met1-Glu159) was expressed.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for GADD45G recombinant protein
Background: GADD45G, also known as CR6, is part of the nuclear proteins to interact with various proteins whose transcript levels are raised after stressful growth arrest conditions and treatment with DNA-damaging agents. GADD45G reacts to environmental stresses by mediating activation of the p38/JNK pathway which is mediated through their protein binding and activating MTK1/MEKK4 kinase, which is an upstream activator of both p38 and JNK MAPKs. GADD45G acts as a new-age tumor suppressor however is being frequently inactivated epigenetically in multiple tumors. GADD45G mRNA expression is down-regulated in hepatocellular carcinoma. GADD45G causes cell cycle arrest at G2/M transition when transfected into Hep-G2 cells. GADD45G induction by androgens involves new protein synthesis. Overexpression of GADD45G inhibits cell growth and causes morphological modifications in prostate cell lines thus GADD45G takes part in differentiation induction by androgens.
Product Categories/Family for GADD45G recombinant protein
References
Takekawa M. et al., 1998, Cell. 95 (4): 521-30. Suzuki M. et al., 1999, J Hum Genet. 44 (5): 300-3. Azam N. et al., 2001, J Biol Chem. 276 (4): 2766-74.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,121 Da
NCBI Official Full Name
growth arrest and DNA damage-inducible protein GADD45 gamma
NCBI Official Synonym Full Names
growth arrest and DNA-damage-inducible, gamma
NCBI Official Symbol
GADD45G
NCBI Official Synonym Symbols
CR6; DDIT2; GRP17; GADD45gamma
NCBI Protein Information
growth arrest and DNA damage-inducible protein GADD45 gamma; DDIT-2; GADD45-gamma; gadd-related protein, 17 kD; cytokine-responsive protein CR6; DNA damage-inducible transcript 2 protein
UniProt Protein Name
Growth arrest and DNA damage-inducible protein GADD45 gamma
Protein Family
UniProt Gene Name
GADD45G
UniProt Synonym Gene Names
CR6; DDIT2; DDIT-2
UniProt Entry Name
GA45G_HUMAN
NCBI Description
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta. [provided by RefSeq, Jul 2008]
Uniprot Description
GADD45G: Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK. Belongs to the GADD45 family.
Protein type: Apoptosis; DNA repair, damage; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 9q22.1-q22.2
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding
Biological Process: activation of MAPKK activity; apoptosis; regulation of cell cycle; positive regulation of apoptosis; multicellular organismal development; activation of MAPKKK activity; positive regulation of JNK cascade; response to stress; negative regulation of protein kinase activity; cell differentiation
Research Articles on GADD45G
Similar Products
Product Notes
The GADD45G gadd45g (Catalog #AAA8121678) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Glu15 9. It is sometimes possible for the material contained within the vial of "GADD45G, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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