GNGT1 recombinant protein

GNGT1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121498

• Gene Names: GNGT1; GNG1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: GNGT1; GNGT1 Protein; Human; Recombinant (His Tag); Human GNGT1/GNG1 Protein (His Tag); GNG1 Protein; guanine nucleotide binding protein (G protein); gamma transducing activity polypeptide 1; GNGT1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Pro2-Cys71

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature foem of human GNGT1 (P63211)(Pro 2-Cys 71) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GNGT1 recombinant protein

Background: GNGT1 is a subunit of transducin. Heterotrimeric G proteins consist of alpha, beta, and gamma subunits. They are membrane-bound GTPases that are linked to 7-TM receptors. They function as signal transducers for the 7-transmembrane-helix G protein-coupled receptors. They are involved as a modulator or transducer in various transmembrane signaling systems. G proteins are bound to GDP in the 'off' state. GNGT1 is the gamma subunit of transducin. Ligand-receptor binding results in detachment of the G protein, switching it to an 'on' state and permitting Galpha activation of second messenger signaling cascades. There are several types of Galpha proteins; also, some Gbetagamma subunits have active functions. Gbetagamma coupled to H1 receptors can activate PLA2 and Gbetagamma coupled to M1 receptors can activate KIR channels. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and G protein-effector interaction.

References

Tao L, et al. (1993) Structure of the bovine transducin gamma subunit gene and analysis of promoter function in transgenic mice. Exp Eye Res. 56 (4): 497-507.Yan K, et al. (1996) Differential ability to form the G protein betagamma complex among members of the beta and gamma subunit families. J Biol Chem. 271 (12): 7141-6.Gaudet R, et al. (1999) A molecular mechanism for the phosphorylation-dependent regulation of heterotrimeric G proteins by phosducin. Mol Cell. 3 (5): 649-60.

NCBI and Uniprot Product Information

• NCBI GI #: 11386179
• NCBI GeneID: 2792
• NCBI Accession #: NP_068774.1
• NCBI GenBank Nucleotide #: NM_021955.3
• UniProt Accession #: P63211; O43835; Q08447; Q16026; A4D1H2
• Molecular Weight: 74
• NCBI Official Full Name: guanine nucleotide-binding protein G(T) subunit gamma-T1
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1
• NCBI Official Symbol: GNGT1
• NCBI Official Synonym Symbols: GNG1
• NCBI Protein Information: guanine nucleotide-binding protein G(T) subunit gamma-T1; guanine nucleotide-binding protein G(T) subunit gamma-T1; transducin gamma chain
• UniProt Protein Name: Guanine nucleotide-binding protein G(T) subunit gamma-T1
• UniProt Gene Name: GNGT1
• UniProt Entry Name: GBG1_HUMAN