CRELD1 recombinant protein

CRELD1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121401

• Gene Names: CRELD1; AVSD2; CIRRIN
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CRELD1; CRELD1 Protein; Human; Recombinant (His Tag); Human CRELD1 Protein (His Tag); AVSD2 Protein; CIRRIN Protein; cysteine-rich with EGF-like domains 1; CRELD1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu362

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 30
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CRELD1 (NP_001070883.1)(Met1-Glu362) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CRELD1 recombinant protein

Background: CRELD1 is a transmembrane glycoprotein. Epidermal growth factor(EGF) like domain exists in CRELD1. EGF-like repeats are a class of cysteine-rich domains that mediate interactions between proteins of diverse function. EGF domains are found in proteins that are either completely secreted or have transmembrane regions that tether the protein to the cell surface. CRELD1 contains a 333 amino acid acid (aa) extracellular domain (ECD), two tandem transmembrane segments, and a second ECD of 15 aa. Defects in CRELD1 may cause susceptibility to atrioventricular septal defect type 2 which results in a persistent common atrioventricular canal.

References

Robinson SW, et al. (2003) Missense Mutations in CRELD1 Are Associated with Cardiac Atrioventricular Septal Defects. Am J Hum Genet. 72(4):1047-52.Zatyka M, et al. (2005) Analysis of CRELD1 as a candidate 3p25 atrioventicular septal defect locus (AVSD2). Clin Genet. 67(6):526-8.Stelzl U, et al. (2005) A human protein-protein interaction network: a resource for annotating the proteome. Cell. 122(6):957-68.

NCBI and Uniprot Product Information

• NCBI GI #: 116734855
• NCBI GeneID: 78987
• NCBI Accession #: NP_001070883.1
• NCBI GenBank Nucleotide #: NM_001077415.2
• Molecular Weight: 45,942 Da
• NCBI Official Full Name: cysteine-rich with EGF-like domain protein 1 isoform 2
• NCBI Official Synonym Full Names: cysteine-rich with EGF-like domains 1
• NCBI Official Symbol: CRELD1
• NCBI Official Synonym Symbols: AVSD2; CIRRIN
• NCBI Protein Information: cysteine-rich with EGF-like domain protein 1
• Protein Family: Cysteine-rich with EGF-like domain protein

NCBI Description

This gene encodes a member of a subfamily of epidermal growth factor-related proteins. The encoded protein is characterized by a cysteine-rich with epidermal growth factor-like domain. This protein may function as a cell adhesion molecule. Mutations in this gene are the cause of atrioventricular septal defect. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]