TCL1A recombinant protein

TCL1A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121311

• Gene Names: TCL1A; TCL1
• Purity: >88% as determined by SDS-PAGE
• Synonyms: TCL1A; TCL1A Protein; Human; Recombinant (His Tag); Human TCL1A Protein (His Tag); TCL1 Protein; T-cell leukemia/lymphoma 1A; TCL1A recombinant protein

• Host: E Coli
• Purity/Purification: >88% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala2-Asp114

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human TCL1A (P56279)(Ala 2-Asp 114) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TCL1A recombinant protein

Background: T-cell leukemia/lymphoma protein 1A (abbreviated for TCL1A) is a member of the TCL1 family. TCL1 protooncogene is expressed in CD3^-CD4^-CD8^- precursor T cells and is extinguished at the CD4⁺CD8⁺ stage of thymocyte development. In B cells, TCL1 is first expressed in pro-B cells and remains high in naive mantle zone B cells of peripheral lymphoid tissues. The TCL1 protooncogene is overexpressed in many mature B cell lymphomas, especially from AIDS patients. It has been demonstrated that TCL1 is a powerful oncogene that, when overexpressed in both B and T cells, predominantly yields mature B cell lymphomas. All TCL1 isoforms bind to the Akt pleckstrin homology domain. Both in vitro and in vivo TCL1 increases Akt kinase activity and as a consequence enhances substrate phosphorylation. In vivo, TCL1 stabilizes the mitochondrial transmembrane potential and enhances cell proliferation and survival. It has been shown that TCL1 is a novel Akt kinase coactivator, which promotes Akt-induced cell survival and proliferation.

References

Jarmo Laine, et al. (2000) The Protooncogene TCL1 Is an Akt Kinase Coactivator. Molecular Cell. 6(2): 395-407.Virgilio L, et al. (1994) Identification of the TCL1 gene involved in T-cell malignancies. PNAS December. 91(26): 12530-4.Hoyer KK, et al. (2002) Dysregulated TCL1 promotes multiple classes of mature B cell lymphoma. Proc Natl Acad Sci U S A. 99(22): 14392-7.

NCBI and Uniprot Product Information

• NCBI GI #: 3024699
• NCBI GeneID: 8115
• UniProt Accession #: P56279; Q6IBK7
• Molecular Weight: 114
• NCBI Official Full Name: T-cell leukemia/lymphoma protein 1A
• NCBI Official Synonym Full Names: T-cell leukemia/lymphoma 1A
• NCBI Official Symbol: TCL1A
• NCBI Official Synonym Symbols: TCL1
• NCBI Protein Information: T-cell leukemia/lymphoma protein 1A; oncogene TCL1; oncogene TCL-1; protein p14 TCL1; T-cell lymphoma-1
• UniProt Protein Name: T-cell leukemia/lymphoma protein 1A
• Protein Family: T-cell leukemia/lymphoma protein
• UniProt Gene Name: TCL1A
• UniProt Synonym Gene Names: TCL1; Oncogene TCL1
• UniProt Entry Name: TCL1A_HUMAN

NCBI Description

Overexpression of the TCL1 gene in humans has been implicated in the development of mature T cell leukemia, in which chromosomal rearrangements bring the TCL1 gene in close proximity to the T-cell antigen receptor (TCR)-alpha (MIM 186880) or TCR-beta (MIM 186930) regulatory elements (summarized by Virgilio et al., 1998 [PubMed 9520462]). In normal T cells TCL1 is expressed in CD4-/CD8- cells, but not in cells at later stages of differentiation. TCL1 functions as a coactivator of the cell survival kinase AKT (MIM 164730) (Laine et al., 2000 [PubMed 10983986]).[supplied by OMIM, Jul 2010]

Uniprot Description

TCL1A: Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival. Homodimer. Interacts with AKT1, AKT2 and AKT3 (via PH domain). Interacts with PNPT1; the interaction has no effect on PNPT1 exonuclease activity. Restricted in the T-cell lineage to immature thymocytes and activated peripheral lymphocytes. Preferentially expressed early in T- and B-lymphocyte differentiation. Belongs to the TCL1 family.

Protein type: Oncoprotein; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: endoplasmic reticulum; pronucleus; cell cortex

Molecular Function: protein binding

Biological Process: multicellular organismal development; stem cell maintenance