KIR2DL1 recombinant protein

KIR2DL1 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8121295

• Gene Names: KIR2DL1; NKAT; NKAT1; p58.1; CD158A; KIR221; NKAT-1; KIR-K64
• Purity: >90% as determined by SDS-PAGE
• Synonyms: KIR2DL1; KIR2DL1 Protein; Human; Recombinant (hFc Tag); Human KIR2DL1/CD158a Protein (Fc Tag); CD158A Protein; KIR-K64 Protein; KIR221 Protein; NKAT Protein; NKAT-1 Protein; NKAT1 Protein; p58.1 Protein; XXbac-BCX195L8.1 Protein; XXbac-BPG184J6.7 Protein; killer cell immunoglobulin like receptor; two Ig domains and long cytoplasmic tail 1; KIR2DL1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His245

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His 22
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human KIR2DL1 (NP_055033.2) extracellular domain (Met 1-His 245) was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for KIR2DL1 recombinant protein

Background: Killer cell immunoglobulin-like receptor 2DL1 or KIR2DL1 is an inhibitory Natural Killer cell immunoglobulin-like receptor with two extracellular immunoglobulin domains. KIR2DL1 is a member of the Killer cell immunoglobulin-like receptor family whose members are classified by the number of the extracellular immunoglobulin domains and the length of the cytoplasm domain. KIR2DL1 is a transmembrane glycoprotein expressed by natural killer cells and subsets of T cells. KIR2DL1 down-regulates the cytotoxicity of NK cells upon recognition of specific class I major histocompatibility complex (MHC) molecules on target cells. It has been reported that the KIR2DL1 is bound to its class I MHC ligand, HLA-Cw4. The KIR2DL1-HLA-Cw4 interface exhibits charge and shape complementarity. Specificity is mediated by a pocket in KIR2DL1 that hosts the Lys80 residue of HLA-Cw4. Many residues conserved in HLA-C and KIR2DL receptors make different interactions in KIR2DL1-HLA-Cw4 and a previously reported KIR2DL2-HLA-Cw3 complex. A dimeric aggregate of KIR-HLA-C complexes was observed in one KIR2DL1-HLA-Cw4 crystal.

Product Categories/Family for KIR2DL1 recombinant protein

ITIM/ITAM Immunoreceptors and Related Molecules

References

Fan QR, et al. (2001) Crystal structure of the human natural killer cell inhibitory receptor KIR2DL1-HLA-Cw4 complex. Nature Immunology. 2: 452-60.Wagtmann N, et al. (1995) Molecular clones of the p58 NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains. Immunity. 2 (5): 439-49.Colonna M, et al. (1995) Cloning of immunoglobulin-superfamily members associated with HLA-C and HLA-B recognition by human natural killer cells. Science. 268 (5209): 405-8.

NCBI and Uniprot Product Information

• NCBI GI #: 110611923
• NCBI GeneID: 3802
• NCBI Accession #: NP_055033.2
• NCBI GenBank Nucleotide #: NM_014218.2
• Molecular Weight: 41,360 Da
• NCBI Official Full Name: killer cell immunoglobulin-like receptor 2DL1
• NCBI Official Synonym Full Names: killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1
• NCBI Official Symbol: KIR2DL1
• NCBI Official Synonym Symbols: NKAT; NKAT1; p58.1; CD158A; KIR221; NKAT-1; KIR-K64
• NCBI Protein Information: killer cell immunoglobulin-like receptor 2DL1; CD158 antigen-like family member A; MHC class I NK cell receptor; killer Ig receptor; killer inhibitory receptor 2-2-1; natural killer-associated transcript 1; p58 NK cell inhibitory receptor NKR-K6; p58 NK r
• Protein Family: Killer cell immunoglobulin-like receptor

NCBI Description

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. [provided by RefSeq, Jul 2008]