KIR2DL1 / CD158a recombinant protein

Recombinant Human KIR2DL1 / CD158a Protein (His tag)

Cat. Num. AAA2545385

• Gene Names: KIR2DL1; NKAT; NKAT1; p58.1; CD158A; KIR221; NKAT-1; KIR-K64
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: KIR2DL1 / CD158a; Recombinant Human KIR2DL1 / CD158a Protein (His tag); XXbac-BPG184J6.7; CD158A; KIR-K64; KIR221; NKAT; NKAT1; p58.1; KIR2DL1 / CD158a recombinant protein

• Host: Human Cells
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 348

• Application Notes: The secreted recombinant human KIR2DL1 comprises 235 amino acids and has a predicted molecular mass of 26 kDa. The apparent molecular mass of rhKIR2DL1 is approximately 45 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: His 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for KIR2DL1 / CD158a recombinant protein

Background: Killer cell immunoglobulin-like receptor 2DL1 or KIR2DL1 is an inhibitory natural Killer cell immunoglobulin-like receptor with two extracellular immunoglobulin domains. KIR2DL1 is a member of the Killer cell immunoglobulin-like receptor family whose members are classified by the number of the extracellular immunoglobulin domains and the length of the cytoplasm domain. KIR2DL1 is a transmembrane glycoprotein expressed by natural killer cells and subsets of T cells. KIR2DL1 down-regulates the cytotoxicity of NK cells upon recognition of specific class I major histocompatibility complex (MHC) molecules on target cells. It has been reported that the KIR2DL1 bound to its class I MHC ligand, HLA-Cw4. The KIR2DL1-HLA-Cw4 interface exhibits charge and shape complementarity. Specificity is mediated by a pocket in KIR2DL1 that hosts the Lys80 residue of HLA-Cw4. Many residues conserved in HLA-C and in KIR2DL receptors make different interactions in KIR2DL1-HLA-Cw4 and in a previously reported KIR2DL2-HLA-Cw3 complex. A dimeric aggregate of KIR-HLA-C complexes was observed in one KIR2DL1-HLA-Cw4 crystal.

Description: A DNA sequence encoding the human KIR2DL1 (NP_055033.2) extracellular domain (Met 1-His 245) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 110611923
• NCBI GeneID: 3802
• NCBI Accession #: NP_055033.2
• NCBI GenBank Nucleotide #: NM_014218.2
• UniProt Accession #: P43626; O43470; Q32WE6; Q6IST4
• Molecular Weight: 41,360 Da
• NCBI Official Full Name: killer cell immunoglobulin-like receptor 2DL1
• NCBI Official Synonym Full Names: killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 1
• NCBI Official Symbol: KIR2DL1
• NCBI Official Synonym Symbols: NKAT; NKAT1; p58.1; CD158A; KIR221; NKAT-1; KIR-K64
• NCBI Protein Information: killer cell immunoglobulin-like receptor 2DL1
• UniProt Protein Name: Killer cell immunoglobulin-like receptor 2DL1
• UniProt Gene Name: KIR2DL1
• UniProt Synonym Gene Names: CD158A; NKAT1; NKAT-1; p58 NK receptor CL-42/47.11

NCBI Description

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. [provided by RefSeq, Jul 2008]

Uniprot Description

KIR2DL1: Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis. Interacts with ARRB2. Interacts with PTPN6; the interaction is enhanced by ARRB2. Interacts with PTPN11; the interaction is enhanced by ARRB2. Belongs to the immunoglobulin superfamily.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: immune response; natural killer cell inhibitory signaling pathway; regulation of immune response