PTP4A2 recombinant protein

PTP4A2 Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121271

• Gene Names: PTP4A2; HH13; OV-1; PRL2; HH7-2; PRL-2; PTP4A; HU-PP-1; PTPCAAX2; ptp-IV1a; ptp-IV1b
• Purity: >90% as determined by SDS-PAGE
• Synonyms: PTP4A2; PTP4A2 Protein; Human; Recombinant (GST Tag); Human PRL-2/PTP4A2 Protein (GST Tag); HH13 Protein; HH7-2 Protein; HMT Protein; HNMT-S1 Protein; HNMT-S2 Protein; HU-PP-1 Protein; OV-1 Protein; PRL-2 Protein; PRL2 Protein; ptp-IV1a Protein; ptp-IV1b Protein; PTP4A Protein; PTPCAAX2 Protein; protein tyrosine phosphatase type IVA; member 2; PTP4A2 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 0.15M NaCl, 1mM GSH, pH7.3. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Asn2-Gln167

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the human PTP4A2 isoform 1 (Q12974-1)(Asn 2-Gln 167) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PTP4A2 recombinant protein

Background: PRL-2 (Protein-tyrosine phosphatase of regenerating liver 2), also known as PTP4A2 (Protein tyrosine phosphatase type IVA, member 2), is a member of PTP family and has an important function in controlling cell growth. PRL-2 phosphatases may be multifunctional enzymes with diverse roles in a variety of tissue and cell types. The phosphatase of regenerating liver (PRL) family, comprising PRL-1, PRL-2 and PRL-3, is a group of prenylated phosphatases that are candidate cancer biomarkers and therapeutic targets. PRL-1, PRL-2, and PRL-3 represent a novel class of protein-tyrosine phosphatase with a C-terminal prenylation motif. They are three closely related intracellular enzymes that possess the PTP active site signature sequence CX 5R. The PRL-2 mRNA is elevated in primary breast tumors relative to matched normal tissue, and also dramatically elevated in metastatic lymph nodes compared with primary tumors. PRL-2 plays a role in breast cancer progression. PRL-2 is a pathogenic molecule in hematopoietic malignancies and it has potential as a novel therapeutic target.

Product Categories/Family for PTP4A2 recombinant protein

Phosphatases and Regulators

References

Akiyama S, et al. (2010) PRL-2 increases Epo and IL-3 responses in hematopoietic cells. Blood Cells Mol Dis. 44(4): 209-14.Hardy S, et al. (2010) Overexpression of the protein tyrosine phosphatase PRL-2 correlates with breast tumor formation and progression. Cancer Res. 70(21): 8959-67.Yuan L, et al. (2007) Differential expression and functional constraint of PRL-2 in hibernating bat. Comp Biochem Physiol B Biochem Mol Biol. 148(4): 375-81.Dumaual CM, et al. (2006) Cellular localization of PRL-1 and PRL-2 gene expression in normal adult human tissues. J Histochem Cytochem. 54(12): 1401-12.Zeng Q, et al. (2000) Prenylation-dependent association of protein-tyrosine phosphatases PRL-1, -2, and -3 with the plasma membrane and the early endosome. J Biol Chem. 275(28): 21444-52.

NCBI and Uniprot Product Information

• NCBI GI #: 18104971
• NCBI GeneID: 8073
• NCBI Accession #: NP_536316.1
• NCBI GenBank Nucleotide #: NM_080391.3
• UniProt Accession #: Q12974; O00649; Q15197; Q15259; Q15260; Q15261; A8K9I8; B4DM39; D3DPP0; E9PGJ6; R4GN50
• Molecular Weight: 19,127 Da
• NCBI Official Full Name: protein tyrosine phosphatase type IVA 2 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase type IVA, member 2
• NCBI Official Symbol: PTP4A2
• NCBI Official Synonym Symbols: HH13; OV-1; PRL2; HH7-2; PRL-2; PTP4A; HU-PP-1; PTPCAAX2; ptp-IV1a; ptp-IV1b
• NCBI Protein Information: protein tyrosine phosphatase type IVA 2; PTP(CAAXII); protein tyrosine phosphatase IVA; protein-tyrosine phosphatase 4a2; protein tyrosine phosphatase IVA2; phosphatase of regenerating liver 2; protein-tyrosine phosphatase of regenerating liver 2
• UniProt Protein Name: Protein tyrosine phosphatase type IVA 2
• Protein Family: Protein tyrosine phosphatase
• UniProt Gene Name: PTP4A2
• UniProt Synonym Gene Names: PRL2; PTPCAAX2; PRL-2
• UniProt Entry Name: TP4A2_HUMAN

NCBI Description

The protein encoded by this gene belongs to a small class of the protein tyrosine phosphatase (PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP was found to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity. Overexpression of this gene in mammalian cells conferred a transformed phenotype, which suggested its role in tumorigenesis. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 11, 12 and 17. [provided by RefSeq, Aug 2010]

Uniprot Description

PTP4A2: Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Promotes tumors. Inhibits geranylgeranyl transferase type II activity by blocking the association between RABGGTA and RABGGTB. Belongs to the protein-tyrosine phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 1p35

Cellular Component: early endosome; cytoplasm; plasma membrane

Molecular Function: prenylated protein tyrosine phosphatase activity