LMAN2 recombinant protein

LMAN2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121172

• Gene Names: LMAN2; GP36B; VIP36; C5orf8
• Purity: >95% as determined by SDS-PAGE
• Synonyms: LMAN2; LMAN2 Protein; Human; Recombinant (His Tag); Human LMAN2/VIP36 Protein (His Tag); C5orf8 Protein; GP36B Protein; VIP36 Protein; lectin; mannose binding 2; LMAN2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, pH8.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Asp45-Arg322

• Species: Human
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human LMAN2 extracellular domain (Q12907)(As p45-Arg 322) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LMAN2 recombinant protein

Background: LMAN2 (Lectin, Mannose Binding 2, also known as VIP36) is a Protein Coding gene. This gene encodes a type I transmembrane lectin that shuttles between the endoplasmic reticulum, the Golgi apparatus, and the plasma membrane. The encoded protein binds high mannose type glycoproteins and may facilitate their sorting, trafficking, and quality control. The L-type lectin LMAN2 appears to be specifically required for the accumulation of GPRC5B in the Golgi complex and restriction of GPRC5B transport along the exosomal pathway. This may occur due to interference with the adaptor protein GGA1-mediated trans-Golgi network-to-endosome transport of GPRC5B. A Golgi-traversing pathway for the exosomal release of the cargo protein GPRC5B in which CD2AP facilitates the entry and LMAN2 impedes the exit of the flux, respectively.

References

Nawa D, et al. (2007) Stable interaction of the cargo receptor VIP36 with molecular chaperone BiP. Glycobiology. 17(9): 913-21.Shimada O, et al. (2003) Localization of VIP36 in the post-Golgi secretory pathway also of rat parotid acinar cells. J Histochem Cytochem. 51(8): 1057-63.Hara-Kuge S, et al. (1999) Vesicular-integral membrane protein, VIP36, recognizes high-mannose type glycans containing alpha1--2 mannosyl residues in MDCK cells. Glycobiology. 9(8): 833-9.Fullekrug J, et al. (1999) VIP36 localisation to the early secretory pathway. J Cell Sci. 112 (Pt 17): 2813-21.

NCBI and Uniprot Product Information

• NCBI GI #: 5803023
• NCBI GeneID: 10960
• NCBI Accession #: NP_006807
• NCBI GenBank Nucleotide #: NM_006816
• UniProt Accession #: Q12907; Q53HH1
• Molecular Weight: 40,229 Da
• NCBI Official Full Name: vesicular integral-membrane protein VIP36
• NCBI Official Synonym Full Names: lectin, mannose-binding 2
• NCBI Official Symbol: LMAN2
• NCBI Official Synonym Symbols: GP36B; VIP36; C5orf8
• NCBI Protein Information: vesicular integral-membrane protein VIP36; glycoprotein GP36b; vesicular integral protein of 36 kDa; vesicular integral-membrane protein 36
• UniProt Protein Name: Vesicular integral-membrane protein VIP36
• Protein Family: Vesicular integral-membrane protein
• UniProt Gene Name: LMAN2
• UniProt Synonym Gene Names: C5orf8; VIP36
• UniProt Entry Name: LMAN2_HUMAN