S100A16 recombinant protein

S100A16 Protein, Human, Recombinant

Cat. Num. AAA8120693

• Gene Names: S100A16; AAG13; S100F; DT1P1A7
• Purity: >92% as determined by SDS-PAGE
• Synonyms: S100A16; S100A16 Protein; Human; Recombinant; Human S100A16/S100F Protein; AAG13 Protein; DT1P1A7 Protein; MGC17528 Protein; S100F Protein; S100 calcium binding protein A16; S100A16 recombinant protein

• Host: E Coli
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser103

• Species: Human
• Predicted N Terminal: Met 1
• Tag: Native
• Protein Construction: A DNA sequence encoding the human S100A16 (Q96FQ6)(Met 1-Ser 103) was expressed and purified.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for S100A16 recombinant protein

Background: S100A16 is a member of S100 protein superfamily that carries calcium-binding EF-hand motifs. S100 proteins are cell- and tissue-specific and are involved in many intra- and extracellular processes through interacting with specific target proteins. S100A16 expression was found to be astrocyte-specific. The S100A16 protein was found to accumulate within nucleoli and to translocate to the cytoplasm in response to Ca(2+) stimulation. The homodimeric structure of human S100A16 in the apo state has been obtained both in the solid state and in solution, resulting in good agreement between the structures with the exception of two loop regions. The homodimeric solution structure of human S100A16 was also calculated in the calcium(II)-bound form. Differently from most S100 proteins, the conformational rearrangement upon calcium binding is minor. Immunoprecipitation analysis revealed that S100A16 could physically interact with tumor suppressor protein p53, also a known inhibitor of adipogenesis. Overexpression or RNA interference-initiated reduction of S100A16 led to the inhibition or activation of the expression of p53-responsive genes, respectively. S100A16 protein is a novel adipogenesis-promoting factor and that increased expression of S100A16 in 3T3-L1 adipocytes can have a negative impact on insulin sensitivity.

Product Categories/Family for S100A16 recombinant protein

Calcium-binding Proteins and Related Molecules

References

Sturchler E, et al. (2006) S100A16, a novel calcium-binding protein of the EF-hand superfamily. J Biol Chem. 281(50): 38905-17.Liu Y, et al. (2011) Identification of S100A16 as a Novel Adipogenesis Promoting Factor in 3T3-L1 Cells. Endocrinology. 152(3): 903-11.Babini E, et al. (2011) Structural characterization of human S100A16, a low-affinity calcium binder. J Biol Inorg Chem. 16(2): 243-56.

NCBI and Uniprot Product Information

• NCBI GI #: 17933772
• NCBI GeneID: 140576
• NCBI Accession #: NP_525127.1
• NCBI GenBank Nucleotide #: NM_080388.1
• UniProt Accession #: Q96FQ6; Q5RHS6; A8K439; D3DV52
• Molecular Weight: 11,801 Da
• NCBI Official Full Name: protein S100-A16
• NCBI Official Synonym Full Names: S100 calcium binding protein A16
• NCBI Official Symbol: S100A16
• NCBI Official Synonym Symbols: AAG13; S100F; DT1P1A7
• NCBI Protein Information: protein S100-A16; protein S100-F; aging-associated protein 13; S100 calcium-binding protein A16; aging-associated gene 13 protein
• UniProt Protein Name: Protein S100-A16
• Protein Family: Protein
• UniProt Gene Name: S100A16
• UniProt Synonym Gene Names: S100F
• UniProt Entry Name: S10AG_HUMAN

Uniprot Description

S100A16: Calcium-binding protein. Binds one calcium ion per monomer. Belongs to the S-100 family.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; nucleolus; plasma membrane; cytosol

Molecular Function: protein homodimerization activity; calcium ion binding

Biological Process: response to calcium ion