Histone H1 Recombinant Protein | H1F0 recombinant protein

Histone H1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120581

• Gene Names: H1F0; H10; H1FV
• Purity: >92% as determined by SDS-PAGE
• Synonyms: Histone H1; Histone H1 Protein; Human; Recombinant (His Tag); Human H1F0/Histone H1 Protein (His Tag); CPN60 Protein; GROEL Protein; H10 Protein; H1FV Protein; HLD4 Protein; HSP60 Protein; HSP65 Protein; HSPD1 Protein; HuCHA60 Protein; SPG13 Protein; H1 histone family; member 0; H1F0 recombinant protein

• Host: E Coli
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 600mM NaCl, 1mM DTT, pH8.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys194

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human H1F0 (P07305)(Met 1-Lys 194) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for H1F0 recombinant protein

Background: H1 histone family, member 0 (H1F0) is a member of the H1 histone family of nuclear proteins which are a component of chromatin in eukaryotic cells. It's involved in maintaining the structure of chromatin by packing the "beads on a string" sub-structure into a high order structure. The lysine-rich H1 histone family in mammals includes eleven members. In higher eukaryotes, all H1 variants have the same general structure, consisting of a central conserved globular domain and less conserved N-terminal and C-terminal tails. These tails are moderately conserved among species, but differ among variants, suggesting a specific function for each H1 variant. Studies on the role of particular subtypes at specific developmental stages in lower eukaryotes, but also in vertebrates suggest that specific subtypes of H1 participate in particular systems of gene regulation.

References

Ramakrishnan V, et al. (1993) Crystal structure of globular domain of histone H5 and its implications for nucleosome binding. Nature. 362 (6417): 219-23.Happel N, et al. (2009) Histone H1 and its isoforms: contribution to chromatin structure and function. Gene. 431 (1-2): 1-12.Izzo A, et al. (2008) The histone H1 family: specific members, specific functions. Biol Chem. 389 (4): 333-43.

NCBI and Uniprot Product Information

• NCBI GI #: 4885371
• NCBI GeneID: 3005
• NCBI Accession #: NP_005309.1
• NCBI GenBank Nucleotide #: NM_005318.3
• UniProt Accession #: P07305; Q6FG88; Q8N6R3; B2R6I0; B4DRD6
• Molecular Weight: 20,863 Da
• NCBI Official Full Name: histone H1.0
• NCBI Official Synonym Full Names: H1 histone family, member 0
• NCBI Official Symbol: H1F0
• NCBI Official Synonym Symbols: H10; H1FV
• NCBI Protein Information: histone H1.0; histone H1'; histone H1(0); H1.0, H1(0), H1-0
• UniProt Protein Name: Histone H1.0
• Protein Family: Histone
• UniProt Gene Name: H1F0
• UniProt Synonym Gene Names: H1FV
• UniProt Entry Name: H10_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. [provided by RefSeq, Jul 2008]

Uniprot Description

H1F0: Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. Belongs to the histone H1/H5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; Golgi apparatus; nuclear chromatin; nucleosome; nucleus; actin cytoskeleton

Molecular Function: protein binding; chromatin DNA binding

Biological Process: nucleosome assembly; apoptosis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis