CAR/CXADR Recombinant Protein | CXADR recombinant protein

CAR/CXADR Protein, Human, Recombinant (His & hFc Tag)

Cat. Num. AAA8120489

• Gene Names: CXADR; CAR; HCAR; CAR4/6
• Purity: >92% as determined by SDS-PAGE
• Synonyms: CAR/CXADR; CAR/CXADR Protein; Human; Recombinant (His & hFc Tag); Human CXADR/CAR Protein (His & Fc Tag); CAR Protein; CAR4/6 Protein; CXADR Protein; HCAR Protein; coxsackie virus and adenovirus receptor; CXADR recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly237

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 20
• Tag: C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the human CXADR (NP_001329.1) extracellular domain (Met 1-Gly 237) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CXADR recombinant protein

Background: CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 17 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 9% sequence identity with the corresponding regions of rat, human CXADR.

References

Tomko, R.P. et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94 (7): 3352-3356. van Raaij, M.J. et al., 2001, Structure. 8 (11): 1147-1155. Cohen, C.J. et al., 2001, J. Biol. Chem. 276 (27): 25392-25398. Carson, S.D. et al., 2002, Rev. Med. Virol. 11 (4): 219-226. Selinka, H.C. et al., 2004, Med. Microbiol. Immunol. 193 (2-3): 127-131. Philipson, L. et al., 2004, Curr. Top. Microbiol. Immunol. 273:87-111. Raschperger, E. et al., 2006, Exp. Cell Res. 312: 1566-1580.

NCBI and Uniprot Product Information

• NCBI GI #: 4503173
• NCBI GeneID: 1525
• NCBI Accession #: NP_001329.1
• NCBI GenBank Nucleotide #: NM_001338.4
• UniProt Accession #: P78310; O00694; Q8WWT6; Q8WWT7; Q8WWT8; Q9UKV4; B2R8V8; B7WPI3; D3YHP0
• Molecular Weight: 35,570 Da
• NCBI Official Full Name: coxsackievirus and adenovirus receptor isoform 1
• NCBI Official Synonym Full Names: coxsackie virus and adenovirus receptor
• NCBI Official Symbol: CXADR
• NCBI Official Synonym Symbols: CAR; HCAR; CAR4/6
• NCBI Protein Information: coxsackievirus and adenovirus receptor; HCVADR; CVB3-binding protein; coxsackievirus B-adenovirus receptor; 46 kD coxsackievirus and adenovirus receptor (CAR) protein
• UniProt Protein Name: Coxsackievirus and adenovirus receptor
• Protein Family: Coxsackievirus and adenovirus receptor
• UniProt Gene Name: CXADR
• UniProt Synonym Gene Names: CAR; CAR; hCAR
• UniProt Entry Name: CXAR_HUMAN

NCBI Description

The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq, May 2011]

Uniprot Description

CXADR: a component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1 located in the plasma membrane of PMN.

Protein type: Membrane protein, integral; Receptor, misc.; Cell adhesion

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: extracellular space; neuron projection; protein complex; tight junction; integral to plasma membrane; basolateral plasma membrane; apicolateral plasma membrane; acrosome; extracellular region; intercellular junction; lipid raft; nucleoplasm; adherens junction; growth cone; plasma membrane; neuromuscular junction; cell junction; filopodium

Molecular Function: viral receptor activity; integrin binding; identical protein binding; protein binding; beta-catenin binding; cell adhesion molecule binding; PDZ domain binding; receptor binding

Biological Process: mitochondrion organization and biogenesis; neutrophil chemotaxis; intercellular junction assembly and maintenance; entry of virus into host cell; regulation of immune response; heart development; gamma-delta T cell activation; heterophilic cell adhesion; cardiac muscle fiber development; actin cytoskeleton reorganization; germ cell migration; homotypic cell-cell adhesion; blood coagulation; defense response to virus; leukocyte migration