Immunofluorescence (IF)
(Immunofluorescence staining of Human CXADR in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CXADR monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.)
Rabbit anti-Human CAR/CXADR Monoclonal Antibody | anti-CXADR antibody
Recombinant Anti-CAR/CXADR Antibody, Rabbit Monoclonal
FC/FACS/FCM: 1:25-1:100
ELISA (Det): 1:1000-1:10,000
ELISA (Det): This antibody will detect Human CXADR/CAR in ELISA pair set.
In a sandwich ELISA, it can be used as detection antibody when paired with.
Immunofluorescence (IF)
(Immunofluorescence staining of Human CXADR in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CXADR monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.)
Immunofluorescence (IF)
(Immunofluorescence staining of Human CXADR in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CXADR monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CXADR expression on HT-29 cells. Cells were stained with purified anti-Human CXADR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CXADR expression on HT-29 cells. Cells were stained with purified anti-Human CXADR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq, May 2011]
Uniprot Description
CXADR: a component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1 located in the plasma membrane of PMN.
Protein type: Membrane protein, integral; Receptor, misc.; Cell adhesion
Chromosomal Location of Human Ortholog: 21q21.1
Cellular Component: extracellular space; neuron projection; protein complex; tight junction; integral to plasma membrane; basolateral plasma membrane; apicolateral plasma membrane; acrosome; extracellular region; intercellular junction; lipid raft; nucleoplasm; adherens junction; growth cone; plasma membrane; neuromuscular junction; cell junction; filopodium
Molecular Function: viral receptor activity; integrin binding; identical protein binding; protein binding; beta-catenin binding; cell adhesion molecule binding; PDZ domain binding; receptor binding
Biological Process: mitochondrion organization and biogenesis; neutrophil chemotaxis; intercellular junction assembly and maintenance; entry of virus into host cell; regulation of immune response; heart development; gamma-delta T cell activation; heterophilic cell adhesion; cardiac muscle fiber development; actin cytoskeleton reorganization; germ cell migration; homotypic cell-cell adhesion; blood coagulation; defense response to virus; leukocyte migration
Research Articles on CXADR
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Product Notes
The CXADR cxadr (Catalog #AAA8103936) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CAR/CXADR Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CAR/CXADR can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA) (Det), Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:20-1:100 FC/FACS/FCM: 1:25-1:100 ELISA (Det): 1:1000-1:10,000 ELISA (Det): This antibody will detect Human CXADR/CAR in ELISA pair set. In a sandwich ELISA, it can be used as detection antibody when paired with. Researchers should empirically determine the suitability of the CXADR cxadr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAR/CXADR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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