Angiopoietin-4 Recombinant Protein | ANGPT4 recombinant protein

Angiopoietin-4 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120170

• Gene Names: ANGPT4; AGP4; ANG4; ANG-3
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Angiopoietin-4; Angiopoietin-4 Protein; Human; Recombinant (hFc Tag); Human Angiopoietin 4/ANG4/ANGPT4 Protein (Fc Tag); AGP4 Protein; ANG-3 Protein; ANG4 Protein; angiopoietin 4; ANGPT4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met282-Ile503

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human ANGPT4 (Q9Y264) C-terminal fragment (Met 282-Ile 503) was fused with the Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

References

Grosios K, et al. (1999) Assignment of ANGPT4, ANGPT1, and ANGPT2 encoding angiopoietins 4, 1 and 2 to human chromosome bands 20p13, 8q22.3q23 and 8p23.1, respectively, by in situ hybridization and radiation hybrid mapping. Cytogenet. Cell Genet. 84 (1-2): 118-20.Yamakawa M, et al. (2004) Expression of angiopoietins in renal epithelial and clear cell carcinoma cells: regulation by hypoxia and participation in angiogenesis. Am J Physiol Renal Physiol. 287 (4): 649-57.Oliner J, et al. (2004) Suppression of angiogenesis and tumor growth by selective inhibition of angiopoietin-2. Cancer Cell. 6 (5): 507-16.

NCBI and Uniprot Product Information

• NCBI GI #: 7705276
• NCBI GeneID: 51378
• NCBI Accession #: NP_057069.1
• NCBI GenBank Nucleotide #: NM_015985.2
• UniProt Accession #: Q9Y264; Q5TFF4; Q9H4Z4
• Molecular Weight: 56,849 Da
• NCBI Official Full Name: angiopoietin-4
• NCBI Official Synonym Full Names: angiopoietin 4
• NCBI Official Symbol: ANGPT4
• NCBI Official Synonym Symbols: AGP4; ANG4; ANG-3
• NCBI Protein Information: angiopoietin-4; ANG-4; angiopoietin-3; dJ824F16.2 (angiopoietin 4)
• UniProt Protein Name: Angiopoietin-4
• Protein Family: Angiopoietin
• UniProt Gene Name: ANGPT4
• UniProt Synonym Gene Names: ANG3; ANG4; ANG-4; ANG-3
• UniProt Entry Name: ANGP4_HUMAN

NCBI Description

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The mechanism by which they contribute to angiogenesis is thought to involve regulation of endothelial cell interactions with supporting perivascular cells. The protein encoded by this gene functions as an agonist and is an angiopoietin. [provided by RefSeq, Jul 2008]

Uniprot Description

ANGPT4: Binds to TEK/TIE2, modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2. Promotes endothelial cell survival, migration and angiogenesis.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: extracellular space; extracellular region

Molecular Function: transmembrane receptor protein tyrosine kinase activator activity; receptor tyrosine kinase binding

Biological Process: negative regulation of angiogenesis; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; transmembrane receptor protein tyrosine kinase activation (dimerization); endothelial cell proliferation; positive regulation of blood vessel endothelial cell migration; angiogenesis; blood coagulation; negative regulation of blood vessel endothelial cell migration; leukocyte migration; negative regulation of apoptosis