MD1 Recombinant Protein | LY86 recombinant protein

MD1 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120134

• Gene Names: LY86; MD1; MD-1; MMD-1; dJ80N2.1
• Purity: >85% as determined by SDS-PAGE
• Synonyms: MD1; MD1 Protein; Human; Recombinant (hFc Tag); Human LY86/MD-16 Protein (Fc Tag); dJ80N2.1 Protein; MD-1 Protein; MMD-1 Protein; lymphocyte antigen 86; LY86 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser162

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 21
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human LY86 (O95711)(Met1-Ser162) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LY86 recombinant protein

Background: MD-1 and MD-2 are secretory glycoproteins that exist on the cell surface in complexes with transmembrane proteins. MD-1 is anchored by radioprotective 15 (RP15) which is a molecule containing leucine-rich repeats and is expressed on B cells, dendritic cells, and macrophages, while MD-2 is associated with TLR4. MD-1 is required for efficient RP15 cell surface expression and function. It is indicated that the RP15/MD1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B cells, and also plays a role in protecting against apoptosis, B-cell proliferation, etc. Mouse MD-1 cDNA encodes a 162 amino acid precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 4% and 66% amino acid sequence identity with chicken and human MD-1 respectively. MD-1 is mainly expressed in the spleen, and also detectable in the liver, brain, thymus, and kidney.

References

Miura Y., et al.,(1998), RP105 is associated with MD-1 and transmits an activation signal in human B cells. Blood 92:2815-2822.Begum N.A., et al., (1999), Human MD-1 homologue is a BCG-regulated gene product in monocytes: Its identification by differential display.Biochem. Biophys. Res. Commun. 256:325-329.Mungall A.J., et al.,(2003), The DNA sequence and analysis of human chromosome 6.Nature 425:805-811.

NCBI and Uniprot Product Information

• NCBI GI #: 4758708
• NCBI GeneID: 9450
• NCBI Accession #: NP_004262.1
• NCBI GenBank Nucleotide #: NM_004271.3
• UniProt Accession #: O95711; Q9UQC4
• Molecular Weight: 17,906 Da
• NCBI Official Full Name: lymphocyte antigen 86
• NCBI Official Synonym Full Names: lymphocyte antigen 86
• NCBI Official Symbol: LY86
• NCBI Official Synonym Symbols: MD1; MD-1; MMD-1; dJ80N2.1
• NCBI Protein Information: lymphocyte antigen 86; MD-1, RP105-associated; ly-86; protein MD-1
• UniProt Protein Name: Lymphocyte antigen 86
• Protein Family: Lymphocyte antigen
• UniProt Gene Name: LY86
• UniProt Synonym Gene Names: MD1; Ly-86
• UniProt Entry Name: LY86_HUMAN

Uniprot Description

LY86: May cooperate with CD180 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) and cytokine production. Important for efficient CD180 cell surface expression.

Protein type: Secreted; Apoptosis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p25.1

Cellular Component: extracellular space; plasma membrane

Biological Process: cell proliferation; apoptosis; positive regulation of lipopolysaccharide-mediated signaling pathway; innate immune response; inflammatory response; humoral immune response