HARS/HisRS Recombinant Protein | HARS recombinant protein

HARS/HisRS Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120051

• Gene Names: HARS; HRS; USH3B
• Purity: >90% as determined by SDS-PAGE
• Synonyms: HARS/HisRS; HARS/HisRS Protein; Human; Recombinant (His Tag); Human HARS Protein (His Tag); HRS Protein; USH3B Protein; histidyl-tRNA synthetase; HARS recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 200mM NaCl, pH7.5, 20% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala2-Cys509

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human HARS (NP_002100.2)(Ala2-Cys509) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6996014
• NCBI GeneID: 3035
• NCBI Accession #: NP_002100.2
• NCBI GenBank Nucleotide #: NM_002109.5
• UniProt Accession #: P12081; B4DHQ1; B4DY73; D6REN6; J3KNE5
• Molecular Weight: 54,847 Da
• NCBI Official Full Name: histidine--tRNA ligase, cytoplasmic isoform 1
• NCBI Official Synonym Full Names: histidyl-tRNA synthetase
• NCBI Official Symbol: HARS
• NCBI Official Synonym Symbols: HRS; USH3B
• NCBI Protein Information: histidine--tRNA ligase, cytoplasmic; HisRS; histidine translase
• UniProt Protein Name: Histidine--tRNA ligase, cytoplasmic
• Protein Family: Histidine--tRNA ligase
• UniProt Gene Name: HARS
• UniProt Synonym Gene Names: HRS; HisRS
• UniProt Entry Name: SYHC_HUMAN

NCBI Description

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

HARS: Defects in HARS are a cause of Usher syndrome type 3B (USH3B). USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life. Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: Translation; EC 6.1.1.21; Ligase

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: cytoplasm; cytosol

Molecular Function: histidine-tRNA ligase activity; ATP binding

Biological Process: tRNA aminoacylation for protein translation; translation; gene expression; histidyl-tRNA aminoacylation

Disease: Usher Syndrome, Type Iiib