Galectin 3/LGALS3 Lysate | LGALS3 lysate

Human Galectin 3/LGALS3 HEK293 Overexpression Lysate

• Applications: Western Blot
• Synonyms: Galectin 3/LGALS3; Human Galectin 3/LGALS3 HEK293 Overexpression Lysate; Human Galectin 3/LGALS3 HEK293 Cell Lysate (WB positive control); Human CBP35 Overexpression Lysate; Human GAL3 Overexpression Lysate; Human GALBP Overexpression Lysate; Human GALIG Overexpression Lysate; Human L31 Overexpression Lysate; Human LGALS2 Overexpression Lysate; Human MAC2 Overexpression Lysate; lectin; galactoside-binding; soluble; 3; LGALS3 lysate

• Host: HEK293 Cells
• Form/Format: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.; 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).

• Applicable Applications for LGALS3 lysate: Western Blot (WB)
• Species: Human
• Sequence Information: A DNA sequence encoding the human LGALS3 (NP_002297.2)(Ala2-Ile250) was expressed with a polyhistidine tag at the C-terminus.
• Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Recommend Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.; 2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.; 3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.
• Preparation and Storage: Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.

Related Product Information for LGALS3 lysate

Description: This Human Galectin 3/LGALS3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Galectin 3/LGALS3 protein from the overexpression lysate was verified.

Background: LGALS3 (Galectin 3) is a Protein Coding gene. This gene encodes a member of the galectin family of carbohydrate-binding proteins. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. LGALS3 is a beta-galactoside-binding lectin and plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion, and T-cell regulation. LGALS3 has an important role in tumor progression through inhibition of apoptosis. LGALS3 expression is associated with neoplastic transformation and with differentiation of monocytes to macrophages. Elevated expression of LGALS3 has been demonstrated in the synovium of rheumatoid arthritis (RA). Diseases associated with LGALS3 include Follicular Adenoma and Papillary Carcinoma.

References

Dumic J, et al. (2006) Galectin-3: an open-ended story. Biochim Biophys Acta. 1760(4): 616-35.Sharma UC, et al. (2004) Galectin-3 marks activated macrophages in failure-prone hypertrophied hearts and contributes to cardiac dysfunction. Circulation. 110(19): 3121-8.Yan YP, et al. (2009) Galectin-3 mediates post-ischemic tissue remodeling. Brain Res. 1288: 116-24.