Influenza A H5N1 ELISA Antibody Pair | H5N1 elisa antibody pair

Influenza A H5N1 (Avian Flu) Hemagglutinin/HA ELISA Pair Set

Cat. Num. AAA8119620

• Reactivity: H5N1
• Synonyms: Influenza A H5N1; Influenza A H5N1 (Avian Flu) Hemagglutinin/HA ELISA Pair Set; Harvey rat sarcoma viral oncogene homolog; H5N1 elisa antibody pair

• Reactivity: H5N1
• Specificity: Quantitative determination of H5N1 Hemagglutinin/HA

• Assay Type: Solid Phase Sandwich ELISA
• Assay Range: 125-8000pg/ml
• Sensitivity: 125pg/ml
• Product Overview: This Influenza A H5N1 (Avian Flu) Hemagglutinin/HA ELISA Pair Set is a solid phase sandwich ELISA for quantitative determination of H5N1 Hemagglutinin/HA. It contains H5N1 Hemagglutinin/HA capture antibody, H5N1 Hemagglutinin/HA detector antibody (HRP) and a highly purified recombinant H5N1 Hemagglutinin/HA protein. This Pair Set is at affordable price for researchers.
• Materials Provided: 1. Capture Antibody 1mg/mL of mouse anti-H5N1 hemagglutinin monoclonal antibody. Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2mg/mL biotinylated rabbit anti-H5N1 hemagglutinin polyclonal antibody. Dilute to a working concentration of 0.8ug/mL in detection antibody dilution buffer before use.; 3. Standard Each vial contains 300ng of recombinant H5N1 hemagglutinin. Reconstitute with 1 mL detection antibody dilution buffer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 8000pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Related Product Information for H5N1 elisa antibody pair

Background: The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

References

White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018