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Western Blot (WB)
(Anti-Carbonic Anhydrase IV rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse lung tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:37 kDa (We are unsure as to the identity of these extra bands.))
Rabbit anti-Mouse Carbonic Anhydrase IV Polyclonal Antibody | anti-CA4 antibody
Anti-Carbonic Anhydrase IV Antibody, Rabbit Polyclonal
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
Carbonic Anhydrase IV; Polyclonal Antibody; Anti-Carbonic Anhydrase IV Antibody; Rabbit Polyclonal; Carbonic Anhydrase IV/Car4 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-AW456718 Antibody; Anti-Ca4 Antibody; Anti-Car4 Antibody; Anti-RP23-167D6.1 Antibody; carbonic anhydrase IV; anti-CA4 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse Car4
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid. 0.2um filtered solution in PBS
Applicable Applications for anti-CA4 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:500-1:3000
IP: 1-5uL/mg of lysate
IHC-P: 1:500-1:3000
IP: 1-5uL/mg of lysate
Immunogen
Recombinant Mouse Car4 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Car4 (rM Car4; NP_031633.1; Met1-Ser277). Car4 specific IgG was purified by Mouse Car4 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Western Blot (WB)
(Anti-Carbonic Anhydrase IV rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse lung tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:37 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-Carbonic Anhydrase IV rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse lung tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:37 kDa (We are unsure as to the identity of these extra bands.))
Immunohistochemistry (IHC)
(Immunochemical staining of mouse Carbonic Anhydrase IV in mouse kidney with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse Carbonic Anhydrase IV in mouse kidney with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse Carbonic Anhydrase IV in mouse lung with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse Carbonic Anhydrase IV in mouse lung with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(mCar4 was immunoprecipitated using: Lane A:0.5 mg Mouse lung Whole Cell Lysate Lane B:0.5 mg A-431 Whole Cell Lysate 4 uL anti- mCar4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- mCar4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37kDa Observed band size :37 kDa)
Immunoprecipitation (IP)
(mCar4 was immunoprecipitated using: Lane A:0.5 mg Mouse lung Whole Cell Lysate Lane B:0.5 mg A-431 Whole Cell Lysate 4 uL anti- mCar4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- mCar4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37kDa Observed band size :37 kDa)
Related Product Information for anti-CA4 antibody
Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.
References
Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1): 1-20.Baird TT, et al. (1997) Catalysis and Inhibition of Human Carbonic Anhydrase IV. Biochemistry. 36 (9): 2669-78.
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Product Notes
The CA4 (Catalog #AAA8119569) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Carbonic Anhydrase IV Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic Anhydrase IV can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:500-1:3000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the CA4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase IV, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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