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Testing Data

COVID 19 Nucleocapsid (NP) Coronavirus Recombinant Protein | COVID-19 recombinant protein

SARS-CoV-2 (2019-nCoV) Nucleocapsid-His Recombinant Protein

Purity
>90 % as determined by SDS-PAGE.
Synonyms
COVID 19 Nucleocapsid (NP) Coronavirus; SARS-CoV-2 (2019-nCoV) Nucleocapsid-His Recombinant Protein; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Nucleocapsid; Nucleoprotein (NP); COVID-19 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
>90 % as determined by SDS-PAGE.
Form/Format
Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Sequence Positions
426aa
Sequence
Met1-Ala419(335Gly/Ala)
Species
2019-nCoV
Tag
N-His
Predicted N Terminal
Met
Protein Construction
|A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (YP_009724397.2) (Met1-Ala419(335Gly/Ala)) was expressed with a polyhistidine tag at the N-terminus.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data
Related Product Information for COVID-19 recombinant protein
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a tool.
References
1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6. Li W. et al., 2005, Science. 309 (5742): 1864-8.

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47.08 kDa
NCBI Official Full Name
nucleocapsid phosphoprotein

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Product Notes

The COVID-19 (Catalog #AAA8118809) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 426aa. The amino acid sequence is listed below: Met1-Ala41 9(335Gly/A la). It is sometimes possible for the material contained within the vial of "COVID 19 Nucleocapsid (NP) Coronavirus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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