IFN-beta Recombinant Protein | IFNB1 recombinant protein

IFN-beta Protein, Human, Recombinant

Cat. Num. AAA8118646

• Gene Names: IFNB1; IFB; IFF; IFNB
• Purity: >95 % as determined by SDS-PAGE
• Synonyms: IFN-beta; IFN-beta Protein; Human; Recombinant; Human Interferon beta/IFN-beta/IFNB Protein; interferon; beta 1; fibroblast; IFB Protein; IFF Protein; IFNB Protein; Interferon beta Protein; IFNB1 recombinant protein

• Host: CHO Stable Cells
• Purity/Purification: >95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 10mM NaAC, 40mM Arg, 0.12mg/ml Met, 146mg/ml Sucrose, 0.1% PF-68, pH4.5 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 166aa
• Sequence: Met1-Asn187

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met22
• Protein Construction: |A DNA sequence encoding the human IFNbeta (P01574) (Met1-Asn187) was expressed
• Activity: Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus. The ED50 for this effect is typically 2-20 pg/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus. The ED50 for this effect is typically 2-20 pg/mL.)

Related Product Information for IFNB1 recombinant protein

Interferons (IFNs) are natural glycoproteins belonging to the cytokine superfamily and are produced by the cells of the immune system of most vertebrates in response to challenges by foreign agents such as viruses, parasites, and tumor cells. Interferon-beta (IFN beta) is an extracellular protein mediator of host defense and homeostasis. IFN beta has well-established direct antiviral, antiproliferative, and immunomodulatory properties. Recombinant IFN beta is approved for the treatment of relapsing-remitting multiple sclerosis. The recombinant IFN beta protein has the theoretical potential to either treat or causes autoimmune neuromuscular disorders by altering the complicated and delicate balances within the immune system networks. It is the most widely prescribed disease-modifying therapy for multiple sclerosis (MS). Large-scale clinical trials have established the clinical efficacy of IFN beta in reducing relapses and slowing disease progression in relapsing-remitting MS. IFN beta therapy was shown to be comparably beneficial for opticospinal MS (OSMS) and conventional MS in Japanese. IFN beta is effective in reducing relapses in secondary progressive MS and may have a modest effect in slowing disability progression. In addition to the common antiviral activity, IFN beta also induces increased production of the p53 gene product which promotes apoptosis and thus has a therapeutic effect against certain cancers. The role of IFN-beta in bone metabolism could warrant its systematic evaluation as a potential adjunct to therapeutic regimens of osteolytic diseases. Furthermore, IFN beta might play a beneficial role in the development of chronic progressive CNS inflammation.

References

Kohriyama T, et al. (2008) Interferon-beta treatment for multiple sclerosis and predictors of response. Nippon Rinsho. 66(6): 1119-26.
Stbgen JP. (2009) Recombinant interferon-beta therapy and neuromuscular disorders. J Neuroimmunol. 212(1-2): 132-41.
Abraham AK, et al. (2009) Mechanisms of interferon-beta effects on bone homeostasis. Biochem Pharmacol. 77(12): 1757-62.

NCBI and Uniprot Product Information

• NCBI GI #: 4504603
• NCBI GeneID: 3456
• NCBI Accession #: NP_002167.1
• NCBI GenBank Nucleotide #: NM_002176.2
• UniProt Accession #: P01574
• Molecular Weight: 22,294 Da
• NCBI Official Full Name: interferon beta
• NCBI Official Synonym Full Names: interferon, beta 1, fibroblast
• NCBI Official Symbol: IFNB1
• NCBI Official Synonym Symbols: IFB; IFF; IFNB
• NCBI Protein Information: interferon beta; IFN-beta; fibroblast interferon
• UniProt Protein Name: Interferon beta
• Protein Family: Interferon
• UniProt Gene Name: IFNB1
• UniProt Synonym Gene Names: IFB; IFNB; IFN-beta
• UniProt Entry Name: IFNB_HUMAN

Uniprot Description

IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: extracellular space; extracellular region

Molecular Function: interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; response to virus; regulation of MHC class I biosynthetic process; natural killer cell activation; negative regulation of T cell differentiation; humoral immune response; T cell activation during immune response; cell surface receptor linked signal transduction; response to exogenous dsRNA; negative regulation of viral genome replication; B cell differentiation; defense response to bacterium; positive regulation of innate immune response; natural killer cell activation during immune response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein; B cell activation during immune response