ICOS ligand ELISA Pair Set | ICOSLG elisa pair set

ICOS ligand Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118381

• Synonyms: ICOS ligand; ICOS ligand Matched ELISA Antibody Pair Set; Human; Human ICOS Ligand/B7-H2/ICOSLG ELISA Pair Set; inducible T-cell co-stimulator ligand; B7-h2 Matched ELISA Antibody Pair Set; B7H2 Matched ELISA Antibody Pair Set; B7RP-1 Matched ELISA Antibody Pair Set; B7RP1 Matched ELISA Antibody Pair Set; CD275 Matched ELISA Antibody Pair Set; GL50 Matched ELISA Antibody Pair Set; ICOS-L Matched ELISA Antibody Pair Set; ICOSL Matched ELISA Antibody Pair Set; ICOSLG Matched ELISA Antibody Pair Set; LICOS Matched ELISA Antibody Pair Set; ICOSLG elisa pair set

• Specificity: Quantitative determination of Human ICOS ligand

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 62.5pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of mouse anti-Human ICOS Ligand/B7-H2/ICOSLG monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of mouse anti-Human ICOS Ligand/B7-H2/ICOSLG monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.25ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 82 ng of recombinant Human ICOS Ligand/B7-H2/ICOSLG. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for ICOSLG elisa pair set

Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. The present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production, and germinal center formation.

This ICOS ligand Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human ICOS ligand. It contains Human ICOS ligand capture antibody, Human ICOS ligand detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human ICOS ligand protein.

Product Categories/Family for ICOSLG elisa pair set

Cancer Drug Targets

References

Flesch IE. (2002) Inducible costimulator-ligand (ICOS-L). J Biol Regul Homeost Agents. 16(3): 217-9.Kajiwara K, et al. (2009) Expression and function of the inducible costimulator ligand B7-H2 in human airway smooth muscle cells. Allergol Int. 58(4): 573-83.Wong SC, et al. (2009) Functional hierarchy and relative contribution of the CD28/B7 and ICOS/B7-H2 costimulatory pathways to T cell-mediated delayed-type hypersensitivity. Cell Immunol. 256(1-2): 64-71.